, 11 tweets, 20 min read Read on Twitter
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 I don't know how I forgot about this study. Very relevant to this discussion
ncbi.nlm.nih.gov/m/pubmed/10856…
It includes details on cholesterol esterification, intracellular TG accumulation and ApoB secretion.
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 ApoB secretion correlated more with intracellular TG accumulation than with cholesterol esterification.
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 Cholesterol esterification was greatest for linoleic (C18:2), linolenic (C18:3), stearic (C18:0) and vaccenic (C18:1 trans).
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 Out of all the fatty acids, only stearic (18:0), vaccenic (18:1, trans) and linolenic (18:3), which didn't promote ApoB secretion.
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 The most oxidizable saturated fatty acids, C12 and C14, are the only fatty acids which didn't promote triglyceride accumulation, and caused the least ApoB secretion.
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 The results of this in vitro study raises two questions:

1-Why does C14 and sometimes C12 appear potent in clinical feeding studies, despite being so weak in promoting ApoB secretion in vitro?
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 2-Why does unsaturated fats, C18:1 and C18:2, both promote ApoB secretion more than does SFA in vitro, yet both decrease ApoB in clinical feeding studies?
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 To answer the first question;
C14 and C12 probably increase LDL-C by other mechanisms different from C16, or probably, these fatty acids are elongated in vivo.

The second question, why does unsaturated greatly increase ApoB in vitro, but lower it in vivo?
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 Probably because unsaturated fatty acids are more likely to be stored in adipocytes rather than being exported to the liver, as opposed to SFA, which is less likely to be stored in adipocytes and more likely to be exported to the liver.
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 I think this is how SFA does increase LDL and M/PUFA decrease it.
@puddleg @MorganPfiffner @DaveKeto @kirbz83 @DoctorTro @MMAdailyfeed @martijnkatan @Karageorgos15 Or probably, because these fatty acids are exported to the liver at different rate -not like this in vitro study- allowing unsaturated fats to be oxidized in the liver, rather than being transported via VLDLs as hypothesized by Katan
Missing some Tweet in this thread?
You can try to force a refresh.

Like this thread? Get email updates or save it to PDF!

Subscribe to Ahmed
Profile picture

Get real-time email alerts when new unrolls are available from this author!

This content may be removed anytime!

Twitter may remove this content at anytime, convert it as a PDF, save and print for later use!

Try unrolling a thread yourself!

how to unroll video

1) Follow Thread Reader App on Twitter so you can easily mention us!

2) Go to a Twitter thread (series of Tweets by the same owner) and mention us with a keyword "unroll" @threadreaderapp unroll

You can practice here first or read more on our help page!

Follow Us on Twitter!

Did Thread Reader help you today?

Support us! We are indie developers!


This site is made by just three indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3.00/month or $30.00/year) and get exclusive features!

Become Premium

Too expensive? Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal Become our Patreon

Thank you for your support!