Getting to watch Dan Turner on LamPORE for #nanoporeconf explain how LamPORE works. Reminds us about how LAMP works - isothermal (just heat, dont heat cycle) and make insane amounts of DNA (micrograms) ("explosive amplification")
LAMP traditionally coupled to a colour change (of flourescent primers nearby) but these have false positives (weird dimers) and dodgy "mid colour" changes. Rather than using fluorophore, ligate nanopore primers, and then put on a nanopore
LAMP gives multiple copies of the target into a long read, and it can have a 10nt bar code. Easy dual bar code, but also the first "LAMP" barcode is *in the LAMP tube*. By having a beta-actin internal control to distinguish "negatives" from "failed reaction" / "failed swab"
Very impressive ROC curve from Dan - as close to a right angle triangle as you might like. On real samples - Ct 19 (easy) to 36 (hard). Assay being validated by external collaborators.
Future: Aiming for folding in adaptive sampling for key regions for host response (!) (this is using the magic from @mattloose)
Aiming to remove sample extraction bottleneck - move from swabs to saliva - inactivate viruses first and then LAMP works (brill!) and have a respiratory panel - a multiplex primers across many primers. Synthetic coinfected mixture works.
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