This thread is to shed a bit of light on the mysteries of how much spike protein is being made, where it is going and how is it going there. Sadly our captured regulatory agencies did not insist the mfr do so
1/ I start by estimating how much spike protein is being created by the mRNA vaccines
2/We know there is 30 microgram for Pfizer
3/Molecular weight is estimated at > 1.25 x10^6 gm/mole. (~330 gm per mole of nucleotide)
This comes out to
30 x 10^-6 grams/
1.25 x 10^6 gm/mole
=24 x 10^-12 moles
6 x 10^23 molecules/mole x 24 x 10^-12 moles =14.4x 10^12 molecules mRNA (14.4trillion)
4/14.4 trillion spike protein if 1 mRNA produces 1 spike protein
A more reasonable number is 1000 spike per mRNA
X1000 14,400 trillion spikes
book.bionumbers.org/how-many-prote…
5/So basically 30 micrograms of mRNA will theoretically produce about 14,400 trillion individual spike proteins (if 100% efficient)
6/Now we ask this. How many cells are transfected by the vaccines. In other words, how many mRNA molecules are in each LNP
7/Since we can estimate how many mRNA molecules there are (14.4 trillion for Pfizer), we must estimate how many LNP’s
8/First we must estimate the size of LNP
“Together with the mRNA, these components form particles of about 60–100 nm in size by using a rapid mixing production technique (Evers et al., 2018).”
9/So lets call it 80 nm and assume a spherical shape.
10/So LNP particles of 80 nm have a total weight of about 0.5 mg. Lipids are typically less dense than water but using the density of water we may estimate the volume of 0.5 mg as 5 x 10^17 cubic nm
sciencedirect.com/science/articl…
11/
An individual 80 nm dia LNP particles volume is 256,000 cubic nm or 2.6 x 10^5 cubic nm/particle
5 x 10^17 volume /2.6 x 10^5 volume/particle =
2 x 10 ^12 LNP particles
And we already estimated
14,400 x 10^12 spike
14.4 x 10^12 mRNA
10^12 is 1 trillion (big number)
12/so basically each LNP will hold about 7 mRNA molecules. And at most 2 x 10^12 cells will be transfected, although its likely multiple LNP will transfect a cell. We just have no way to estimate this
13/Current estimates of cells in human body are 37 x10^12, and we have 2 x 10^12 LNP and 14.4 x 10^ 12 mRNA that will produce up to 14,400 x 10^12 spike protein
14/Obviously these are ballpark numbers. Its a shame we don’t have precise numbers on these questions and so many others such where does the mRNA end up and how long before its degraded
15/ Its simply incredible to me that they were not required to do a biodistribution study on primates yo find out where the mRNA goes to after its injected.
16/Pfizer used a luciferase mRNA but only tracked the lipids in the LNP. Moderna used a study on a different mRNA and lipids . Both of them used mice
ncbi.nlm.nih.gov/pmc/articles/P…
16a/Using the Moderna study we see mRNA is widely distributed but in small amounts. However, if we remember that mRNA produces roughly 1000 times its weight in protein then maybe its not as insignificant as we think
17/The other thing we want to know is where does the spike protein end up. We know that once produced in the cell its presented on the cell surface (and some is chopped up and presented on MHC-I or MhC-2 molecules depending on the cell type)
link.springer.com/article/10.100…
18/We know that the cells transfected and which produce spike protein are destroyed by the immune cells or the cells undergo apoptosis or necrosis, and the idea is that the protein is completely destroyed
19/ yet we also have seen studies showing the S1 protein in the plasma of nurses after vaccination for up to 28 days after vaccination, and also the entire spike protein for a shorter period
academic.oup.com/cid/advance-ar…
20/ We have also seen studies on mice showing S1 can disrupt the blood brain barrier and pass into the brain, and in vitro human models
pubmed.ncbi.nlm.nih.gov/33053430/
nature.com/articles/s4159…
21/ Furthermore Dr Patterson has discovered that S1 persists in non-classical monocytes for up to 15 months after infection and is seeing the same in post-vaccination patients.
22/These monocytes repair blood vessel wall and crawl over the blood vessel and may be causing inflammation which cause long haul symptoms
23/The question for all that is how an IM injection can lead to LNP/mRNA or spike proteins into the circulatory system. Some Doctors say its not possible, they are too big but don’t mention Transcytosis
researchgate.net/publication/66…
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