The least-easy-to-remember hashtag on twitter appears to have gained steam. If you can't remember the whole thing just look for the #modernagate hashtag and you should find the other one #CTCCTCGGCGGGCACGTAG
dailymail.co.uk/news/article-1…
A quick recap because there are so many people on this platform covering for Moderna...
This is the GenBank entry in the patent database
Note the main author - S. Bancel (CEO of Moderna) who just said his "experts" are "looking into it" 🤔
Here is the BLAST of #CTCCTCGGCGGGCACGTAG against LZ959695.1 (the Moderna entry)
You can reproduce it with this link (but expires in 24 hr) blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=…
As explained in the paper the sequence in the Moderna patent is the reverse complement of the viral sequence. It's what you would find on the complementary strand of DNA.
So it's CTACGTGCCCGCCGAGGAG.
Here it is in the Genbank seq here (permalink) ncbi.nlm.nih.gov/nuccore/LZ9596…
You can copy and paste the whole sequence (3387nt long) into expasy.org/translate to see the amino acids.
In the 5'3' direction (how the mammalian cell would translate the sequence) you don't see PRRAR
But in the complementary direction, which could happen in the situation described in the Frontiers paper, you get this:
Which is the PRRAR (furin cleavage site) you're interested in, if you're looking for one.
#CTCCTCGGCGGGCACGTAG
Here is the BLASTn comparison of the Moderna mutated MSH3 (LZ959695.1) to human MSH3 (NM_002439.5), showing homology and the changes.
It appears that there are a lot of genomic changes in this sequence but this is due to codon optimisation, there are in fact very few changes to the protein sequence.
Here is the protein BLAST, showing the magic - an almost identical sequence but with a deletion
You can reproduce this yourself by doing a BLASTp search on the amino acid sequence of the Moderna MSH3 aligned to NP_002430.3 (the protein sequence of human MSH3)
blast.ncbi.nlm.nih.gov/Blast.cgi?PROG…
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