Wreck the KG anderson “data”.




RnYN02 is almost 1/10 human(the 3’-ETS not found in bats or non-humans).
No evidence of an insertion event ever happening in RmYN02. FauI utility is RFLP and HR1 cloning.
Esp3I site and BamHI sites in RaTG13 is entirely different from CoV2, and is far harder to clone with no see’ms technology. Could still be Esp3I, since you can cut it out.

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More from @flavinkins

23 Sep
Shredding a "paper" who uses multiple fraudulent "evidence" to "debunk" the idea that SARS-CoV-2 is not a natural zoonosis.
This "paper" was made by K_G Anderson, the creater of the fraudulent O-linked glycan claim to defend the WIV.
Claim 1: "e. SARS-CoV-1 and Middle East respiratory syndrome (MERS) have furin cleavage sites in their Spike protein." There are no furin cleavage sites in SARS-CoV-1, and MERS-CoV is a DPP4-using merbecovirus that have entirely different tropism and dynamics than Sarbecoviruses. Image

an absence of furin cleavage sites were found in Sarbecoviruses, the only ACE2-using beta-CoVs.
Read 48 tweets
22 Sep
In 2018, experiments with a novel recombinant SARS coronavirus substituted the typical TRS transcription leader and body sequences with a novel sequence (UGGUCGC) in an attempt to further reduce recombination in animal models as a live vaccine candidate (nature.com/articles/s4200…).
This TRS leader sequence, supposedly novel, is found starting at nucleotide 1465 in SARS-CoV-2 and could result, if utilized, in a novel viral RNA transcript that deletes part of the nsp2 protein of the ORF 1ab polyprotein.
It is also found at nucleotide 1446 in RaTG13, one of the viruses found in the Yunnan cave in 2013, which has been proposed as a precursor to SARS-CoV-2. It is found in that area in no other coronavirus. RaTG13 is fraudulent. Can’t infect a bat. The SRA is garbage.
Read 6 tweets
20 Sep


How Odd this EK1 is! Specific, confirmed, highly potent fusion inhibitor against CoV2. Online since early 2019??! Why Shi mentioned it as early as in Jan 31? Why not other drugs? Vaccines? Anything else? ImageImageImage
This was also the ZC45/ZXC21 homology paper. They have 4991 high connection—why never mentioned RaTG13? @WlKD6iuxiZqBvm7 @HimalayaGlobal @G_Translators
Read 4 tweets
20 Sep
@GentleMutt @BidoliNicola @MonaRahalkar @BillyBostickson @angoffinet @TheSeeker268 zenodo.org/record/4008432…
The real issue is that the raw data itself does not support the existence of that assembly. The rest of the SRA is a complete mess. Everything that have a match to a bat matches the genomic DNA instead of the mRNA. There were no bacterial reads which is
@GentleMutt @BidoliNicola @MonaRahalkar @BillyBostickson @angoffinet @TheSeeker268 Impossible for a “fecal swab”—every fecal sample on NCBI, if you search “bat feces” on SRA, with the Bacterial reads reduced below 5% in total cellular organisms, lacked any (confirmed) reads that can be traced to an RNA virus. There are other reads, including nearly 60% of a
@GentleMutt @BidoliNicola @MonaRahalkar @BillyBostickson @angoffinet @TheSeeker268 Non-homopolymer repeat sequence, which have no known biological mean of origin. It preprints.org/manuscript/202… have only ever been observed in significant quantities in 2 other datasets—the first one is a completely destroyed dataset (from tissue samples) with one amplicon of a
Read 19 tweets
19 Sep
CRISPR gene editing to chop off the first 9 exons of ACE2, so only the short isoform remained, that can’t bind to CoV2 any more? @ch_zimmer
This isoform is a Carboxypeptidase and have ACE2 activity. So it will still work. It is no longer a receptor.
And will no longer allow infection.
Read 15 tweets
19 Sep
And every other part of that “raw data” points to complete RNA degradation—and complete virus destruction. Go to NCBI and search “bat feces”. Everything that had <5% bacteria lacked any virus—traced and confirmable through BLAST.
The only set of amplicons that shared a well with the others in the same direction, possessed unusually low query coverage and appear to be in-house synthesized.
In fact, nearly all the bat “RNA” were partial match to the refseq RNA—indicative of the source of match being genomic DNA, filtered using a refseq/InSDC system and then separately merged into the dataset. The “bat mRNA” matches were always partial matches. If a clone is present,
Read 6 tweets

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