Inside and Outside of kids cloth mask worn all day at school.
Plated on PDA with no selection.

Central planning and it’s effect on evolution and anti-fragility?
If 7B people all wear 300um filters that collect microbes, the human microbiome will change. Is this Good or bad? ImageImage
In order for one to identify the microbes. ITS/16S sequencing or whole genome sequencing is required.

We describe that in these manuscripts. Image
Cannabis microbiomes are relevant to the mask debate as this is the study of microbes you might inhale and thus we are particularly sensitized to fungi that can cause aspergillosis or fusariosis.

Microbes that synthesize mycotoxins are important as well. Image
When immunocompromised patients get hit with Aspergillosis...
50% fatality rate.

Tad higher than C19.

Remington tracks this back to a cannabis vaporizer.

Vaporizers use nebulization to create Cannabinoid aerosols. Not hot enough to sterilize… Image
This is just the tip of the iceberg.
Most microbes (~95%) we don’t know how to culture so only ~5% of the diversity shows up on PDA.

In many ways Culture is just performance art or microbiome tarot cards.

The cool kids sequence.… Image
Sequence not only identifies the microbe but it can inform you if it has genes that synthesize mycotoxins.
Citrinin and Paxilline genes were discovered this way. These are mycotoxins found in Penicillium that can negatively impact seizure control with Cannabidiol. Image
You can run the exp. at your school. PDA plates can be bought from Teknova.
Just stamp your mask for a minute onto a plate. Remove mask and Seal the lid and let it grow at RT for 3-4 days.

Everyone’s will be a little different and kids can all make fun of each other’s bugs. Image
I would clean the masks after running the experiment..obviously.
I think teachers should engage students over this new and odd behavior we’ve mandated.

Teaching science is important but If schools fail to teach human rights, they are just indoctrination camps.

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More from @Kevin_McKernan

13 Nov
What happens when you use RNA polymerase mutagens in your fight against a virus?

Life Finds a way. Mutations accelerate.…
I’m just speculating on this. This is a great read on the types of polymerase errors this modified nucleotide tends to create.

Keep in mind the patient was on a lot of drug including HCQ so this one patient study won’t answer this. Image
From the supplement.
Poor soul had a rough fight. Image
Read 4 tweets
13 Nov
This genome was Crypto funded and Crypto peer reviewed.
Project published in 5 months.

Decentralized funding and peer review seemed crazy in 2018. If you recall Surgisphere, you might appreciate censorship resistant science.
We presented on this Crypto Incentivized Blockchain recorded Review (CIBR) concept @TexasBitcoin in 2018. It foreshadows much the censorship and distortion of science seen in C19.

We propose as solution that introduces price signals and free markets.

Many people cringe at $ in peer review.
Meanwhile Journal publication prices continue to rise while digital content distribution costs fall.

The $ in peer review should go to the people doing the most valuable work (review). Free work generates errors.…
Read 5 tweets
13 Nov
Freedom Requires Live-Dead analysis: Subgenomic RNA

You need to pay attention to Wolfel et al and Kim et al.

A very elegant method for Live-Dead analysis.

But before we dive into that Mol-Bio hole, you must see the gravity of Liotti’s work.… Image
Liotti (&Wolfel) demonstrate that your infectious stage is above 1-2M RNA copies/ml and usually lasts 5 days. However non-infectious RNA can last 77 days. To be safe they suggest 10 days and 100,000 copies/ml as non-infectious. Image
So what is creating the long tail of qPCR positivity that is not infectious.

This is believed to be due to subGenomic RNA. Kim et al has the best review on this.… Image
Read 19 tweets
11 Nov
In Mass over 85-90% of the tests are CQ >30.
John conflates this with the 1:2000 FP rate specified in my tweet which he didn’t read because he was too busy verify GBD signatories.

CVs are seasonal. Its expected their viral loads go down in the summer.…
Other CVs seasonality.
I’d love an explanation for how a virus does this and maintains constant CQ across the population.

I won’t hold my breath as I’m a GBD signatory and that makes me a conspiracy theorist that needs remote mocking as opposed to a normal scientific discussion
A normal person would ask a question on your thread.
“have you considered X?”

A toxic person will take your work out of context, mis-represent it and then use an associative GBR fallacy to mock you while barely scraping the surface of what you’ve said.

Not science. Politics
Read 8 tweets
11 Nov
Schrodinger’s CQ

I asked many labs if they could share CQ data. The Broad Institute responded immediately with: possible but requires some work.

Another lab became defensive, threatened to litigate and tweeted me “a horrible person” for probing

How did we get here? Lessons?
Lesson #1) everyone is stressed out and getting hit with questions over FPs and conflicts. I’m guilty of pointing out that testing labs have revenue interests and higher positive tests create more contact map testing.
Underscoring this COI doesn’t make friends. I will do better.
Lesson 2) Resistance to public CQ was mostly defended by demanding evidence they have a FP problem.

“Prove it before we show you.”

I was probably the 100th person since the NYTimes article that hit them up on this and I see why they were short.
Read 13 tweets
2 Nov
Are PFU/Cq curves perfect?
They are the best we have today.

The pioneering work that looked at this by Jaafar et al. Used Monkey cells or Vero cells.

Now let’s look at the work at the CDC culturing the virus.…
Vero cells were chosen as they were the most sensitive to forming plaques.
Aka the virus was very good at killing these cells.
But the CDC was thorough. They didn’t just look at Vero cells. They looked at many other cell lines including human cell lines.

Have a look at Figure 3.

Virus isn’t as virulent to other cell lines. I say virulent because they are measuring Viral titres as PFU.
Plaques are dead.
Read 5 tweets

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