This Story begins with Cancer and Ends with Psilocybe cubensis.

It is a very timely topic given the lockdown induced suicide, anxiety and depression.

In fact, Psilocybin has been awarded FDA breakthrough status for these very things.
medscape.com/viewarticle/92…
Johns Hopkins has looked into this in Terminal Cancer Depression.
archpsyc.jamanetwork.com/article.aspx?a…
thelancet.com/pb/assets/raw/…

It is time to take a deep dive
but first the Cancer story

In 2016, I became a green thumb on a mission. My father had stage4 bone mets with BRAF:K601E mutations
While cannabinoids were unmatched by western medicine for cancer related cachexia, pain and nausea, there was an added hopeful kicker; Cannabinoid were non-toxic potent AKT1 inhibitors. Just what the Foundation medicine report called for ...except they had to stick to FDA drugs.
The Drugs for AKT1 inhibitors were all in phase 2 and 3 small trials with different tumor types. Not much promise there.
But the pathway was obvious.
I grew my 1st type II cannabis plants, learned hydro, made extracts, and PCR and HPLC QC’d them. He was immunocompromised. Microbial contamination was our main concern. Cross state trustworthy supply was limited to CBD, CBDA, and CBG by law.
This is where people think we're nuts.
More links to assure you we are not.
The pathway has many in vitro models. The drugs are non-toxic and are the only thing that allowed him to Eat. But our supply for THCA needed to be local due to federal and state law conflict.
I knew too much about the microbial contam occurring in the state of Mass as they largely adopted culture based microbial regulations which are all voodoo. Most pathogenic microbes dont culture. Particularly the ones effecting Immunocompromised patients.
pubmed.ncbi.nlm.nih.gov/26100839/
I had to grow it myself for THCA and THC.
This turned into the Jamaican Lion genome project, recently honored as one of the most significant advances in the cannabis industry in 2020.
thecannabisscientist.com/business-profe…
It was legal to do this Mass but there was always the federal risk which complicated every trip to the hospital and to their home in NH. Drug dogs visited their home in NH. Imagine dying of cancer and frisks. The police kindly turned a blind eye once they saw the circumstances.
This in and of itself is a wild and amazing story and I encourage anyone interested to read this tribute to his amazing life and our journey through this process here:
medicinalgenomics.com/tribute-to-ric…
When you perform a deep dive on cannabinoids and cancer, it’s hard not to run into the JHUs work on terminal cancer depression and psilocybin and while my Father never needed this, it never left my radar as sometimes family members are equally effected but such trying times.
Another medicinal genome that was left un-sequenced due to prohibition and it was a small target. I could do it in a weekend and assemble it on my laptop.

So I did.
But I was afraid to publish it. I was my father’s caretaker. A raid would end his cannabinoid supply and while a remote fear, it would have devastating consequences. This was the time frame when Ross Ulbricht @Free_Ross was being persecuted for allegedly hosting the Silk Road.
Double life sentence + 40 years for hosting a website where users traded mostly cannabis and mushrooms.

How does one publish a genome anonymously in 2016?

This is where I began to see the ugly choke hold of journals on the dissemination of scientific information.
I discovered this is not easy. Any website would have ties back to a user acct. Blockchains came to mind but the systems for blockchain file storage was nascent. IPFS was exciting but hard to index and search. The genome remained difficult to find on esoteric corners of the web.
Another group (Fricke et al) published a beautiful genome and pathway characterization in 2017. We sequenced a different one in 2016 and have aligned that to Fricke et al and placed it public for everyone to surf.
SNPs in the key genes involved in synthesis of Psilocybin, Psilocin, Baeocystin,NorBaeocystin, Aeruginascin may provide a deeper understanding of the entourage effects in Cubensis.
With Oregons’ legalization and the FDA declaring Psilocybin a Break-Through therapy, it is time to blow the dust off this genome.

Here it is. Open source. send it around.
genomevolution.org/coge/GenomeInf…
There are 120K-280K SNPs between the two genomes (depending on the SNP caller). About a SNP every 160 bases. Notice the High impact variants in PsiK, the gene responsible for Phosphorylating the molecule from Psilocin into Psilocybin.
Variants in the Methyltransferase are equaling interesting as they may govern the synthesis of Baeocystin, norBaeocystin and Aeruginascin.
Why genomics? These are very difficult molecules to synthesize in the lab and as a result a genomics based approach is likely to lead to the most productive dissection of the pathways and variants related to the chemotype diversity in P.Cubensis. Fricke has done much of this work
We need to end the drug war and free the prisoners like @FreeRoss that are caged up over this for 2X life. This is a war on science and on people. It is impacting very powerful compounds that can address many of the comorbidities seen in C19 and the depressing reaction to it.
If you want to learn more about cannabinoids and cancer, come to CannMed 2021. @CannMedEvents

The Videos from the last 4 years of the conference are open source and online.
cannmedevents.com/2019-videos/

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More from @Kevin_McKernan

21 Nov
10M PCR tests ending in August and zero evidence of asymptomatic spread.

I think I recall Fauci saying this exact thing at the start of all this.

Who convinced you otherwise?
A few excerbs ...
Kids and teachers rates are amount the lowest.

It’s as if it’s Opposite Day in the central planners office.

Stuff C19 into nursing homes and torture the least at risk population in schools?

All reinfections were non-infectious on viral culture.
Read 15 tweets
21 Nov
When asked “where did flu go?”

Having run a CLIA lab, you must follow the Gordian knot of CPT codes and liability.
Hospital Acquired Infections (HAI) often fall on the hospital to cover.

Flu has risk of HAI
C19 is liability free.

Admin gonna admin.

ama-assn.org/practice-manag…
It will be interesting to see the adoption of multiplex testing where Flu and C19 are tested in parallel.

A few papers that use these kits see 1:300 co-infection rates.

Now have CPT codes for mux testing.

ama-assn.org/practice-manag…
Assays for multiplex testing are here.

watermark.silverchair.com/jiaa658.pdf?to…
Read 5 tweets
13 Nov
What happens when you use RNA polymerase mutagens in your fight against a virus?

Life Finds a way. Mutations accelerate.
nejm.org/doi/full/10.10…
I’m just speculating on this. This is a great read on the types of polymerase errors this modified nucleotide tends to create.

Keep in mind the patient was on a lot of drug including HCQ so this one patient study won’t answer this.
From the supplement.
Poor soul had a rough fight.
Read 4 tweets
13 Nov
This genome was Crypto funded and Crypto peer reviewed.
Project published in 5 months.

Decentralized funding and peer review seemed crazy in 2018. If you recall Surgisphere, you might appreciate censorship resistant science.
osf.io/7d968/
@saifedean
@JamesTodaroMD
We presented on this Crypto Incentivized Blockchain recorded Review (CIBR) concept @TexasBitcoin in 2018. It foreshadows much the censorship and distortion of science seen in C19.

We propose as solution that introduces price signals and free markets.

Many people cringe at $ in peer review.
Meanwhile Journal publication prices continue to rise while digital content distribution costs fall.

The $ in peer review should go to the people doing the most valuable work (review). Free work generates errors.

medicinalgenomics.com/cryptocurrency…
Read 5 tweets
13 Nov
Freedom Requires Live-Dead analysis: Subgenomic RNA

You need to pay attention to Wolfel et al and Kim et al.

A very elegant method for Live-Dead analysis.

But before we dive into that Mol-Bio hole, you must see the gravity of Liotti’s work.

jamanetwork.com/journals/jamai…
Liotti (&Wolfel) demonstrate that your infectious stage is above 1-2M RNA copies/ml and usually lasts 5 days. However non-infectious RNA can last 77 days. To be safe they suggest 10 days and 100,000 copies/ml as non-infectious.
So what is creating the long tail of qPCR positivity that is not infectious.

This is believed to be due to subGenomic RNA. Kim et al has the best review on this.

ncbi.nlm.nih.gov/pmc/articles/P…
Read 23 tweets
12 Nov
Inside and Outside of kids cloth mask worn all day at school.
Plated on PDA with no selection.

Central planning and it’s effect on evolution and anti-fragility?
If 7B people all wear 300um filters that collect microbes, the human microbiome will change. Is this Good or bad? ImageImage
In order for one to identify the microbes. ITS/16S sequencing or whole genome sequencing is required.

We describe that in these manuscripts.

f1000research.com/articles/5-2471

f1000research.com/articles/4-1422 Image
Cannabis microbiomes are relevant to the mask debate as this is the study of microbes you might inhale and thus we are particularly sensitized to fungi that can cause aspergillosis or fusariosis.

Microbes that synthesize mycotoxins are important as well. Image
Read 9 tweets

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