Is the CASYQTQTNSPRRAR a good vaccine target?
This is the C terminus of the S1 protein. The S1-S2 interaction interface is just upstream of it. This epitope is found in all SARS-CoV-2 isolates that have productive transmission. Target it and it will be forced to become common cold.
Antibodies directed against this peptide will rip the S apart.

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More from @flavinkins

19 Nov
Just as expected, the “GD-1 amplicon sequence” was sequenced from vectors. The pEASY vector allow transcription through T7 and SP6 promoters in-vitro and support gibson assembly for the generation of infectious clones. They can make endless amount of pure RNA to Spike in samples.
(Digest with restriction enzyme and then assemble with NEBuilder HiFi DNA assembly kit).
The clade “Inovirus” was found in several PRJNA640246 datasets. This is the vector backbone.
Read 13 tweets
19 Nov
@SongFei2017 @OshZosh @TheSeeker268 As it turned out, the most critical 1-29 of ACE2 was NOT supported by ANY SRA data. this mean that the "R24 of RaACE2" was created through the use of Yeast Display--and the ZLS "data" was complete fabrication.
Read 10 tweets
19 Nov
drive.google.com/file/d/1rpmEgw…
However, upon closer examination, it revealed that they were comparing against HUMAN ACE2. RaTG13 appeared to have highest infectivity on Mouse ACE2. and "RaACE2" was even poorer for RaTg13 binding than hACE2--meaning NO In-VIVO infection.
drive.google.com/file/d/1rpmEgw…
In addition, a thorough BLAST analysis of all 961 SRA datasets of Rhinolophus on NCBI, including Environmental and multispecies datasets, FAILED to return any evidence of ACE2 1-29--two residues in which are "critical in stabilizing the RaACE2-RBD complex"
If RaTG13 have an RaACE2 affinity even poorer than hACE2, as suggested by their flow cytometry and pseudovirus transduction, then there is NO CHANCE that RaTg13 will even infect the real BAT! (the miners were "NOT" infected by a virus with a NP that is similar to CoV2 or RaTG13).
Read 6 tweets
18 Nov
trace.ncbi.nlm.nih.gov/Traces/sra/?ru…
Appears to be their new “amplicon” sequence.
However, none of the new “amplicons” contained any of the original “GD-1 Amplicon sequence” sequences as their substring. They looks like that they have used

drive.google.com/file/d/1y8yUo_…

drive.google.com/file/d/1pD7xxt…
ncbi.nlm.nih.gov/pmc/articles/P…
To simulate their amplicons. There are a lot of amplicons there with an “M13” on it. They must have emergency sequenced their infectious clone (M13 mean that it have been sequenced from a vector)! Also there were suddenly too many amplicons there.
Where did the first GD_1 sequences came from? They aren’t in the new Ab1 files. Why there were so many sequences labeled “M13” (a sequencing primer. Found in cloning vectors and indicate that these were sequenced from vectors instead of raw cDNA) ? Looks like that they broke
Read 13 tweets

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