➡️Construction of the SARS COV Chimera 2.👇

1) Between the Spike protein SARS COV 2 and human ACE 2 there is a occorre "mediator" = Spike RBD SARS COV 2. ➡️In the silent cloning digest sites, of the genomic amino acid codon, the novel betacoronavirus, lineage B,
2) Spike RBD sequence is inserted. The seed particles plus the SARS-Spike Chimera are placed in a culture, in-vitro, where 👉293T is present. 👉293T is a human cell line, derived from the HEK 293 cell line, which expresses a mutant version of the large SV40 T antigen.
3) 👉 (It is commonly used in biology for the expression of proteins and the 👉production of recombinant retroviruses. Human embryonic kidney 293 cells, often referred to as HEK 293, HEK-293, 293 cells or less precisely as HEK cells, are a specific cell line originally derived
4) from human embryonic kidney 👉cells grown in tissue cultures taken from a female fetus aborted). Once the in-vitro recombination is complete, 👉 of the Pseudotypes are obtained (composed of the sum of seed particles plus the SARS-Spike Chimera), which are then placed in
5) a second culture the Target cells. Finally we get the measure luciferase for human cells entry.👇

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2 Feb
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disclosures contained herein are subject to an AS-IS representation. The author does not bear responsibility for errors in
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