πŸ›‘πŸ›‘πŸ›‘βž‘οΈ Lab BaricπŸ‘‡
"Our mouse adapted #SARSCoV2 strain, MA10, is now available from BEI Resources"πŸ‘‡
➑️A Mouse-Adapted SARS-CoV-2 Induces Acute Lung Injury and Mortality in Standard Laboratory Mice.πŸ‘‡
cell.com/cell/fulltext/…
➑️ Humanized C57BL / 6J mice, produced by Lab Baric and Jefferson Lab,are the most widely used strain.
πŸ‘‰It is commonly used to replicate human tests with highly pathogenic viruses.
πŸ‘‰Its resistance to tumors is known, and varies from the age of the mouse. 1/2
πŸ‘‰For this reason tests are overestimated and therefore not very reliable. There is a black market for transgenic laboratory mice.
➑️ China is one of the biggest buyers ...πŸ‘‡
βž‘οΈπŸ‘‡

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More from @BidoliNicola

7 Feb
πŸ›‘πŸ›‘πŸ›‘βž‘οΈ The results of our 5-year SARSr-CoV surveillance in a cave inhabited by multiple species of horseshoe bats in Yunnan.
πŸ‘‰The Study reveal that the SARSr-CoVs circulating in this single location are highly diverse in the S, ORF3 and ORF8 gene!πŸ‘‡

1)A large number of
2) SARS-related coronaviruses (SARSr-CoV) have been detected in horseshoe bats since 2005 in different areas of China. However, these bat SARSr-CoVs show sequence differences to SARS coronavirus (SARS-CoV) in different genes (S, ORF8, ORF3, etc.). Below we report the results
3) of our 5-year SARSr-CoV surveillance in a cave inhabited by multiple species of horseshoe bats in Yunnan Province, China. The full-length genomes of 11 recently discovered SARSr-CoV strains, along with our previous findings, reveal that the SARSr-CoVs circulating in this
Read 25 tweets
4 Feb
➑️Construction of the SARS COV Chimera 2.πŸ‘‡

1) Between the Spike protein SARS COV 2 and human ACE 2 there is a occorre "mediator" = Spike RBD SARS COV 2. ➑️In the silent cloning digest sites, of the genomic amino acid codon, the novel betacoronavirus, lineage B,
2) Spike RBD sequence is inserted. The seed particles plus the SARS-Spike Chimera are placed in a culture, in-vitro, where πŸ‘‰293T is present. πŸ‘‰293T is a human cell line, derived from the HEK 293 cell line, which expresses a mutant version of the large SV40 T antigen.
3) πŸ‘‰ (It is commonly used in biology for the expression of proteins and the πŸ‘‰production of recombinant retroviruses. Human embryonic kidney 293 cells, often referred to as HEK 293, HEK-293, 293 cells or less precisely as HEK cells, are a specific cell line originally derived
Read 7 tweets
2 Feb
➑️➑️➑️ What happened in BSL 4, by Whuan, in spring 2018 and that Beijing does not want to admit.πŸ‘‡

πŸ‘‰ Let's face it, working in a BSL4 is a continuous risk, for multiple reasons:
- 1) you can prick your fingers if you misuse a syringe; your suit will be contaminated and the virus can enter your circulatory system directly.
- 2) When you undress from the containment suit,after having carried out a double cycle of anti-contaminating showers, you can get dressed in your civilian clothes and put on your personal jewelry or,for example,contact lenses;here the virus can enter through the mucous membranes
Read 21 tweets
1 Feb
@Geraldo22603187 @AlexandraJAlva4 @quay_dr @FLMIRONES ➑️1)an RNA virus can never be extinguished, much less by a vaccine. There are too many variants that the virus can mutate, the coverage spectrum of a messenger mRNA vaccine is too targeted and therefore cannot include future variants. 2) An inactive vaccine may do better than an
@Geraldo22603187 @AlexandraJAlva4 @quay_dr @FLMIRONES mRNA vaccine, but its immunization is too low. 3) Consequently, it is advisable to treat the virus only with specific medicines such as: HCQ, or Invermectin. 4) An RNA virus can, over time, mitigate its infectious virulence, but it CANNOT disappear; we must get used to living
@Geraldo22603187 @AlexandraJAlva4 @quay_dr @FLMIRONES with him. 5) We must ONLY protect frail (elderly) people, due to their weakened immune systems, and we must return to our normal life, as before the chimeric pseudo-pandemic. 6) We must learn to distinguish between proper health care, which really protects the health of the
Read 5 tweets
31 Jan
➑️ SARS COV 2 ORF8πŸ‘‡
πŸ‘‰A large number of SARS-related coronaviruses (SARSr-CoV) have been detected in horseshoe bats since 2005 in different areas of China. However, these bat SARSr-CoVs show sequence differences from SARS coronavirus (SARS-CoV) in different genes (S, ORF8, ORF3,
1) etc. and are considered unlikely to represent the direct progenitor of SARS-CoV. Herein, we report the findings of our 5-year surveillance of SARSr-CoVs in a cave inhabited by multiple species of horseshoe bats in Yunnan Province, China. The full-length genomes of 11 newly
2) discovered SARSr-CoV strains, together with our previous findings, reveals that the SARSr-CoVs circulating in this single location are highly diverse in the S gene, ORF3 and ORF8. Importantly, strains with high genetic similarity to SARS-CoV in the hypervariable N-terminal
Read 11 tweets
31 Jan
➑️ SKP2 InhibitorπŸ‘‡ cell.com/trends/pharmac…
1) In humans, airway epithelial cells highly express the putative SARS-CoV-2 entry receptor, angiotensin-converting enzyme 2 (ACE2) and transmembrane serine proteinase 2 (TMPRSS2), the receptor that the virus uses to prime the S
2) protein (spike protein of SARS-CoV-2) [4]. SARS-CoV-2 infection experiments using primary human airway epithelial cells have been found to have cytopathic effects 96 h after the infection [5]. However, primary human airway epithelial cells are expensive and do not proliferate
3) indefinitelyiv. Several infinitely proliferating cell lines, such as Caco-2 [6], Calu-3 [7], HEK293T [8], and Huh7 [7] have been utilized in SARS-CoV-2 infection experiments. These cell lines do not accurately mimic human physiological conditions and generate low titer of
Read 24 tweets

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