πŸ›‘πŸ›‘πŸ›‘βž‘οΈ The ACE2 / RBD of πŸ‘‰ FELINE (cats) badger even properly bind the S1 of SARS-CoV-2 and have an entry efficiency superior than even a Yunnan bat!!! Image
➑️ Also the Feline Coronavirus (FCoV) and its mutation, FIP (Feline Infectious Peritonitis) matches SARS COV 2.
πŸ‘‰In this study, we demonstrate that stimulation of nsp16 2β€²-O-MTase activity by nsp10 is a universal and conserved mechanism in coronaviruses, 1/4 Image
including FCoV (Feline Coronavirus), and that nsp10 is functionally interchangeable in the stimulation of nsp16 of different coronaviruses. Based on our current and previous studies, we designed a peptide (TP29) from the sequence of the interaction 2/4
interface of mouse hepatitis virus (MHV) nsp10 and demonstrated that the peptide inhibits the 2β€²-O-MTase activity of different coronaviruses in biochemical assays and the viral replication in MHV infection and SARS-CoV replicon models. Interestingly, the peptide TP29 exerted 3/4
robust inhibitory effects in vivo in MHV-infected mice by impairing MHV virulence and pathogenesis through suppressing virus replication and enhancing type I interferon production at an early stage of infection.Therefore, as a proof of principle, the current results indicate 4/4
that coronavirus 2β€²-O-MTase activity can be targeted in vitro and in vivo. πŸ‘‡5/4
ncbi.nlm.nih.gov/pmc/articles/P…
@MartinaSisters , FYIπŸ‘‡ Image
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More from @BidoliNicola

21 Feb
πŸ›‘πŸ›‘πŸ›‘βž‘οΈ Novel Mechanism Chimeric Proteins: πŸ‘‡

➑️ Upstream Frankentension Orf
πŸ‘‰UFO. πŸ‘‡

➑️ 1) In the latest issue of πŸ‘‰Cell (June 25, 2020), Ho et al. 1 report that = the IAV, which belongs to sNSVs, can obtain functional upstream start codons (uAUG) by
2) appropriating 5 β€² terminal mRNA sequences from their hosts, and this mechanism is called πŸ‘‰"snatching", which reflects the characteristic of grabbing codon associated sequences of beginning.
πŸ‘‰In chimeric host viral transcripts, the host-derived translation from uAUG would
3) access upstream viral ORFs (uvORF).
πŸ‘‰I et al. determined the abundance of uAUG in cap-snatch host sequences stored in a DEFEND-seq dataset that was previously generated from IAV-infected A549 cells.
2 These authors observed that the distributions of cap-snatched
Read 8 tweets
16 Feb
➑️ ➑️➑️ IDENTIFICATION OF THE HIGHLY INFECTIVE GENOMIC COMPOSITION OF SARS COV 2 RNA: πŸ‘‡

➑️ 1) SARS COV 2 is a particular chimeric "armored" virus, consisting of external protuberances of S (Spike) coated, externally, by glycans and, inside these protuberances, the TGEV
2) bacteria (E. Coli bacterium, of gastroenteritis) have been identified and HIV retroviruses.

πŸ‘‰The outer membrane M has the TMPRSS2 protein which, associated with ACE2, allows the same protein S to enter the host cells.
This mechanism is well explained
3) by the process of fusion of the M membrane with the plasma membrane of the cell.
(It is important to note that a TMPRSS2 inhibitor is BROMEXINE).

πŸ‘‰The inner nucleus N is instead composed of highly pathogenic viral genomes such as: MVH / HCV (Hepatitis C) an RNA virus
Read 21 tweets
9 Feb
πŸ›‘πŸ›‘πŸ›‘βž‘οΈ Lab BaricπŸ‘‡
"Our mouse adapted #SARSCoV2 strain, MA10, is now available from BEI Resources"πŸ‘‡
➑️A Mouse-Adapted SARS-CoV-2 Induces Acute Lung Injury and Mortality in Standard Laboratory Mice.πŸ‘‡
cell.com/cell/fulltext/…
➑️ Humanized C57BL / 6J mice, produced by Lab Baric and Jefferson Lab,are the most widely used strain.
πŸ‘‰It is commonly used to replicate human tests with highly pathogenic viruses.
πŸ‘‰Its resistance to tumors is known, and varies from the age of the mouse. 1/2
πŸ‘‰For this reason tests are overestimated and therefore not very reliable. There is a black market for transgenic laboratory mice.
➑️ China is one of the biggest buyers ...πŸ‘‡
Read 5 tweets
7 Feb
πŸ›‘πŸ›‘πŸ›‘βž‘οΈ The results of our 5-year SARSr-CoV surveillance in a cave inhabited by multiple species of horseshoe bats in Yunnan.
πŸ‘‰The Study reveal that the SARSr-CoVs circulating in this single location are highly diverse in the S, ORF3 and ORF8 gene!πŸ‘‡

1)A large number of
2) SARS-related coronaviruses (SARSr-CoV) have been detected in horseshoe bats since 2005 in different areas of China. However, these bat SARSr-CoVs show sequence differences to SARS coronavirus (SARS-CoV) in different genes (S, ORF8, ORF3, etc.). Below we report the results
3) of our 5-year SARSr-CoV surveillance in a cave inhabited by multiple species of horseshoe bats in Yunnan Province, China. The full-length genomes of 11 recently discovered SARSr-CoV strains, along with our previous findings, reveal that the SARSr-CoVs circulating in this
Read 25 tweets
4 Feb
➑️Construction of the SARS COV Chimera 2.πŸ‘‡

1) Between the Spike protein SARS COV 2 and human ACE 2 there is a occorre "mediator" = Spike RBD SARS COV 2. ➑️In the silent cloning digest sites, of the genomic amino acid codon, the novel betacoronavirus, lineage B,
2) Spike RBD sequence is inserted. The seed particles plus the SARS-Spike Chimera are placed in a culture, in-vitro, where πŸ‘‰293T is present. πŸ‘‰293T is a human cell line, derived from the HEK 293 cell line, which expresses a mutant version of the large SV40 T antigen.
3) πŸ‘‰ (It is commonly used in biology for the expression of proteins and the πŸ‘‰production of recombinant retroviruses. Human embryonic kidney 293 cells, often referred to as HEK 293, HEK-293, 293 cells or less precisely as HEK cells, are a specific cell line originally derived
Read 7 tweets
2 Feb
➑️➑️➑️ What happened in BSL 4, by Whuan, in spring 2018 and that Beijing does not want to admit.πŸ‘‡

πŸ‘‰ Let's face it, working in a BSL4 is a continuous risk, for multiple reasons:
- 1) you can prick your fingers if you misuse a syringe; your suit will be contaminated and the virus can enter your circulatory system directly.
- 2) When you undress from the containment suit,after having carried out a double cycle of anti-contaminating showers, you can get dressed in your civilian clothes and put on your personal jewelry or,for example,contact lenses;here the virus can enter through the mucous membranes
Read 21 tweets

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