twistbioscience.com/sites/default/…
“KEY HIGHLIGHTS
• Fully synthetic RNA generated from Twist Gene Fragments
• >99.9% viral genome coverage
• NGS sequence verified
• Positive control for both RT-PCR and
NGS-based assays”
That is correct: fully synthetic, 29856nt-long, RNA controls,
“KEY HIGHLIGHTS
• Fully synthetic RNA generated from Twist Gene Fragments
• >99.9% viral genome coverage
• NGS sequence verified
• Positive control for both RT-PCR and
NGS-based assays”
That is correct: fully synthetic, 29856nt-long, RNA controls,
indistinguishable from live virus, BSL-1 and can be used to generate realistic fakes of ANY sequences within weeks. Spike into animal sample, “intermediate host!” Spike into bat samples, “closely related!” Spike into pangolin samples, “pangolin coronavirus!” “Non-overlapping”
By the product sheet? Just order them in Overlapping fragments in stead. GeneFragments and ssRNA transcription service (include DNAse I digestion to clear away the templates, T7 run-off transcription to eliminate terminators) gives realistic RNA spiking forensically
Indistinguishable from real virus. rapidmicrobiology.com/news/fda-recom…
Available since April 2020–make every single dataset made or published after that date highly suspect. twistbioscience.com/products/genes
Available since April 2020–make every single dataset made or published after that date highly suspect. twistbioscience.com/products/genes
“Gene Fragments are linear synthesized DNA sequences for direct cloning or larger gene assembly.”
Linear DNA, no vector, T7 can be included for run-off in-vitro transcription. Up to 5Kb. T7 polymerase just need to have a stretch of “GG” for transcription start and no promoter
Linear DNA, no vector, T7 can be included for run-off in-vitro transcription. Up to 5Kb. T7 polymerase just need to have a stretch of “GG” for transcription start and no promoter
Parts are required to be seen on the final RNA. Digest template with RNAse-free DNAse I after completion, and you get RNA that is indistinguishable from the real thing. Better, order by the TRS segments and use more RNA for the 3’-end of the genome to get even more realistic fake
Datasets.
@DrLiMengYAN1 @ThomasConnors @BillyBostickson @RolandBakerIII @nerdhaspower
NO Trust for anything sequenced after 04/2020!
NO Trust for anything sequenced after 04/2020!
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