➡️➡️➡️ Confirmed high level of identity of the FIPV, and with the SARS COV 2.👇

➡️1) FeCV-FIPV / HCoV-229E ratio; the non-random and non-natural interaction between these two coronaviruses has allowed the development of the SARS CoV 2 Chimera.
➡️ 2) The percentage of identity between FeCV-FIPV, and SARS CoV 2, is 👉44.0-44.5%, but if you use👉 NSP16 as a mediator, things change and you get to an identity percentage of 👉99.6-99 , 9%, between FeCV nsp16, and SARS CoV 2 nsp16.
➡️ 3) Human coronavirus👉 229E is a species of coronavirus that infects humans and 👉bats Alphacoronavirus, of the subgenus Duvinacovirus.
It is a positive-sense, single-stranded RNA virus that enters its host cell by binding to the 👉APN receptor.👇
🛑🛑🛑➡️ 4) But if 👉fAPN, of the Feline Coronavirus👉 FeCV, is used as a👉 mediator, it is possible to introduce both SARS and CoV into human cells, thanks to the use of 👉humanized leukemic mice.

The cells of humanized leukemic mice, once infected, develop viral
5) antigens in the cytoplasm, these immunized cells have shown an identity with👉 human APN of = 78%.

In support of the above, a pre-covid document, dated 1996, explains the relationship found between feline infectious peritonitis (FIPV), feline enteric coronavirus (FeCV).
6) To test whether feline NPC could act as a receptor for two feline serogroup I coronaviruses, feline infectious peritonitis virus (FIPV) and feline enteric coronavirus (FeCV), the authors cloned the cDNA encoding feline NPC (fAPN) using PCR, from cDNA isolated to determine a
7) feline cell line and stably expressed in mouse and hamster cells, resistant to FIPV and FeCV. (The predicted amino acid sequence of fAPN shows 78% identity with human PNA.)

Infection of mouse NIH 3T3 cells, transfected with fAPN with feline and murine coronaviruses, were
8) then inoculated with CCV, porcine coronavirus (TGEV), human coronavirus (HCV-229E), FIPV UCD-1 strain, or murine coronavirus (MHV) -A59.

Viral antigens were detected 6 hours after inoculation of NIH 3T3, transfected with fAPN, with CCV, TGEV, MHV-A59 and after 24
9) hours with HCV-229E, FIPV UCD-1.

Upon subsequent screening, the transfected NIH 3T3 cells did not develop any viral antigens.

However, vector-only transfected NIH 3T3 mouse cells were susceptible to mouse MHV-A59 coronavirus infections as were
10) 👉fAPN-transfected NIH 3T3 cells, since bile glycoprotein-bound receptors for MHV are expressed on these cells.

➡️ Then feline infectious peritonitis virus (FIPV) and feline enteric coronavirus (FeCV) have been shown to be related to human coronavirus HCV-229E.
11) It is no coincidence that SARS COV 2 present, between S1 / S2 and N, the viruses of haemorrhagic gastroenteritis (TGEV), human hepatitis C (HCV) and murine hepatitis (MHV) which proved to be a virus considered a prototype of the CoV.
➡️ 12) In order to further understand the FIPV / SARS-CoV relationship, the relationship with the 👉nsp16 protein needs to be explained.

👉The 5 'cap structures of eukaryotic mRNAs are important for RNA stability and protein translation. Many viruses that replicate in the
13) cytoplasm of eukaryotes have evolved 2′-O-methyltransferases (2′-O-MTases) to autonomously modify their mRNAs and carry a cap-1 structure (m7GpppNm) at the 5 'end, thus facilitating viral replication and escape innate immune recognition in host cells.
14) Previous studies have shown that the 2′-O -MTase activity of non-structural protein 16 (nsp16) of severe acute respiratory syndrome coronavirus (SARS-CoV) must be activated by nsp10, while nsp16 of feline coronavirus (FCoV) by it only
15) possesses 2′-O -MTase activity (E. Decroly et al., J Virol 82: 8071–8084).

👉Thus the stimulation of nsp16 2′-O-The activity of MTase by nsp10 is a universal and conserved mechanism in coronaviruses, including FCoV, and that nsp10 is functionally
16) interchangeable in the stimulation of nsp16 of different coronaviruses. It was also verified whether the P29 peptide could inhibit the replication of the virus in the replication models of the live MHV virus and the SARS-CoV replicon.
18) To deliver the short peptide to the cells, the N-terminus of the P29,P29M,and P29S peptides has been fused with an👉 HIV-Tat short peptide👉 (YGRKKRRQRRRGSG)which is rich in👉arginine and can act as a vector that penetrates cells to deliver the load on the cytosol and
19) the nucleus.

👉The HIV-Tat fused peptides were named TP29, TP29M and TP29S, respectively.

👉Rabbits use CGG for👉 arginine 👉11.4% of the time, exactly the same percentage as humans, compared to 👉4% in bats.

➡️ In an extract from a Paper by
20) Shi Zhengli, we read that due to the very low arginine and little present in bats, through an Elisa test, Shi Zengli used rabbit polyclonal antibodies, exactly 👉Antirabbit IgC, to recombine 👉6 coronaviruses of bats, coming from the caves of Yunnan,
21) allowing a considerable 👉increase in arginine.👇

(Shi Zhengli, jvi.asm.org/content/89/16/… )
22) ➡️➡️➡️ Returning to 👉HCoV-229E it was discovered that this Coronavirus has not been much studied, in fact it not only infects the👉 Alphacoronavirus bat clade but, also, that of 👉Betacoronavirus.

We report a fatal case of COVID-19 pneumonia coinfected
23) with HCoV-229E in Hong Kong.

➡️ Genome sequencing of 👉SARS CoV 2 and 👉HCoV-229E from a patient's nasopharyngeal sample showed that the wild 👉HK13 virus strain of SARS CoV 2 was more closely related to the 👉SARS CoV 2 Wuhan-type strain Hu-1 👉(99.99% nucleotide
24) identity), consistent with his recent history of patient-made trips to👉 Wuhan. 👇

pubmed.ncbi.nlm.nih.gov/33568452/

 ➡️ First case reported in 2018.👇

hindawi.com/journals/criid…
➡️ 25) SARS CoV 2, the causative agent responsible for coronavirus disease 2019 (C19) belongs to the genus Betacoronavirus.

👉Betacoronaviruses that infect canines, felines and other animals are normally expected to have a limited correlation with SARS CoV 2.
26) However, dogs, cats, tigers, lions and minks tested positive for SARS CoV 2 👉(American Veterinary Medical Association 2020, Gollakner & Capua 2020, Halfmann et al. 2020, Wageningen Bioveterinary Research 2020).

The low susceptibility of dogs to SARS CoV 2 infection was
27) confirmed by the x-ray structures of the human host receptor angiotensin 2 (ACE2) converting enzyme bound to the binding domain of the spike protein receptor SARS-CoV-2; dogs displayed relatively low levels of ACE2 in the airways (Zhai al et. 2020).
28) In fact, the canine CRCoV shared a 45.8-46.2% similarity with SARS CoV 2.

👉After sequence alignment using ClustalW in MEGA software and phylogenetic analysis using Splits Tree software, we found that CoVs of animal origin dispersed into separate clades.
29) Feline CoVs shared 👉 44.0-44.5% identity with SARS CoV and SARS CoV 2 at the nucleotide level.

🛑🛑🛑➡️ But if we compare SARS CoV 2 isolates from 👉wild felines (Tigre-MT065033) they are very close to the 👉SARS CoV 2 Human isolates, sharing an identity of ➡️99.6-99.9%.⬅️
30) SARS CoV 2👉 Mink isolates (MT396266) were also very similar to human SARS CoV 2 isolates, sharing an identity of 👉99.6-99.9%.

➡️➡️➡️ This is proof that both sevatic felines and ferrets have been used in in vivo experiments, as intermediate animals, in ACE2
31) (see the cases of the experiments carried out in the BSL4 of 👉Harbin, and of the👉 WIV).

SARS-like bat CoVs (CoVZXC21; MG772934) and bat CoVs (RaTG13; MN996532) ranged from 86.6 to 96.3%.

Furthermore, SARS-CoV and MERS-CoV showed 80.6-81.1%
32) and 51% identity respectively, with SARS-CoV-2 at the nucleotide level.👇

onlinelibrary.wiley.com/doi/full/10.11…
33)FCoV and SARS COV / nsp16, KDA correspondence level👇
34) ➡️➡️➡️ It is very important to remember that the 👉recombinant nsp16 SARS CoV 2 protein finds its maximum expression in 👉E. Coli cells, more precisely, the recombinant SARS CoV 2 NSP16 is produced by the E. coli expression system.
35) Therefore, Coronaviruses, such as SARS COV 2, employ "molecular machines", called proteins, to infect host cells through bacteria and replicate.

👉E. Coli are enterobacteria that find their ideal habitat in the intestines of humans and other animals.
➡️ 36) There are, however, some types of E. coli that have such a 👉pathogenicity that they can cause even very serious diseases, such as 👉enteritis, 👉hemorrhagic colitis,👉 urinary infections, 👉meningitis and 👉septicemia.

👉E. coli is also responsible for
37) extraintestinal infections, such as: 👉Biliary infections;
👉Meningitis;
👉Pneumonia;
👉Peritonitis,
➡️ all pathologies well present in 👉SARS COV 2.

➡️ CONCLUSION:
Summarizing the 👉2′-O -MTase activity of non-structural👉 protein 16 (nsp16) of severe acute
38) respiratory syndrome coronavirus (SARS CoV) must be activated by nsp10, while ➡️nsp16 of feline coronavirus (FCoV) alone👉 2′- O -MTase ➡️activity spontaneous to him natural.

➡️ In fact, if we compare SARS CoV 2 isolates from 👉wild felines (Tiger - MT065033) they appear to
39) be very close to human SARS CoV 2 isolates, sharing an identity of➡️ 99.6-99.9%.⬅️

➡️ This explains why 👉sevatic felines are used in in vivo experiments, as intermediate animals, in ACE2, in BSL4 laboratories (such as Whuan's infamous BSL4 WIV) thanks to the
40) nsp16 protein, present in their 👉feline coronavirus (FCoV), which alone possesses 👉spontaneous 2′- O -MTase activity.

➡️Thus 2′-O methylation is a strategy used by pathogenic viruses to produce mRNA with 5 ′ ends that mimic host cellular
41) mRNAs, consequently ➡️2′-O-MTase⬅️ is a potential target for 👉vaccine development but, at the same time for the 👉GOF principle, also home to the development of a 👉chimeric armored virus!

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3 Apr
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