💥Integration News💥

➡️ Unexpected discovery of multiple coronaviruses and a BSL-3 pathogen in agricultural cotton and rice sequencing dataset.

➡️ Identified a new Merbecovirus, related to HKU5, in a cotton dataset sequenced by Huazhong Agricultural University in 2017.

➡️ We also found a sequence of infectious clones containing a novel HKU4-related Merbecovirus related to the MERS coronavirus in a rice dataset sequenced from Huazhong Agricultural University in early 2020.
➡️ Another HKU5-related Merbecovirus, as well as the Japanese encephalitis virus, were identified in a cotton dataset sequenced by Huazhong Agricultural University in 2018.

➡️ A HKU3-related Betacoronavirus was found in a Mus musculus sequencing dataset from the Wuhan
Institite of Virology in 2017.

➡️ Finally, a SARS-WIV1-like Betacoronavirus was found in a rice dataset sequenced by Fujian Agriculture and Forestry University in 2017.



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More from @BidoliNicola

8 Apr
➡️ Cell reprogramming expressed through the RNA-induced mutagenicity of SARSCoV 2, of the infected cells. 👇
➡️The mechanism:
1)Cellular entry of SARS-CoV-2 depends on the binding of protein S to the angiotensin 2 converting enzyme (ACE2) which is a specific cellular receptor, located on the surface of the host cell, to facilitate zoonotic transfer (these viruses may involve ACE2 from
2) various animal species.)

Beta-coronaviruses replicate in the cytoplasm; Cellular compartments such as the endoplasmic reticulum and the intermediate compartment of the endoplasmic reticulum of the Golgi apparatus undergo intense remodeling.

After the internalization and
Read 36 tweets
4 Apr
➡️ Cell reprogramming expressed through RNA-induced mutagenicity of #SARSCoV2 in infected cells confirmed.👇

1) SARS-CoV-2 is an enveloped beta-Coronavirus RNA belonging to the Coronaviridae family.

The genome is packaged inside a helical capsid formed by the
2) nucleocapsid (N) protein.

Three other structural proteins are associated with the viral envelope: membrane (M), envelope (E) and glycoprotein peak (S).

Cellular entry of SARS-CoV-2 depends on the binding of protein S to the angiotensin converting enzyme 2 (ACE2) which is a
3) specific cell receptor located on the surface of the host cell.

This receptor facilitates zoonotic transfer because these viruses can involve ACE2 from different animal species.

Beta-coronaviruses replicate in the cytoplasm; cellular compartments such as the
Read 47 tweets
3 Apr
@Grace42Thorn @janieyaya @MartinaSisters ➡️1) Probable, not all retroviruses cause cancer, but all RNA tumor viruses are retroviruses.
They transform an infected cell by the action of one or more genes, which are present in the viral genome and are called viral oncogenes...
@Grace42Thorn @janieyaya @MartinaSisters 2) RNA viruses, known as retroviruses, which can induce the neoplastic transformation of eukaryotic cells. Examples are the Rous sarcoma virus, the feline leukemia virus, the avian erythroblastosis virus, etc ...
@Grace42Thorn @janieyaya @MartinaSisters 3) The RNA of the virus is made up of:
👉gag = gene that codes for a precursor protein which, once cut, produces the proteins of the viral particle.
👉pol = gene that codes for reverse transcriptase, an enzyme that has the task of transcribing single-stranded RNA to
Read 20 tweets
1 Apr
➡️➡️➡️ Confirmed high level of identity of the FIPV, and with the SARS COV 2.👇

➡️1) FeCV-FIPV / HCoV-229E ratio; the non-random and non-natural interaction between these two coronaviruses has allowed the development of the SARS CoV 2 Chimera.
➡️ 2) The percentage of identity between FeCV-FIPV, and SARS CoV 2, is 👉44.0-44.5%, but if you use👉 NSP16 as a mediator, things change and you get to an identity percentage of 👉99.6-99 , 9%, between FeCV nsp16, and SARS CoV 2 nsp16.
➡️ 3) Human coronavirus👉 229E is a species of coronavirus that infects humans and 👉bats Alphacoronavirus, of the subgenus Duvinacovirus.
It is a positive-sense, single-stranded RNA virus that enters its host cell by binding to the 👉APN receptor.👇
Read 41 tweets
31 Mar
➡️D614G (Asp 614→Gly) as a replacement in S1, increased the ACE2 affinity, leading to 👉more infectivity and transmissibility.

➡️ There are non-synonymous nucleotide modifications, including 👉E484K, 👉N501Y and / or 👉K417N (Lys 417 → Asn) in the ACE2 interface of the RBD.
➡️ There are also various deletions in the amino (N) -terminal (NTD) domain of S1 in 👉B.1.1.7 and 👉B.1.351.

➡️ Although most mutations in these variants were observed in a smaller fraction of the SARS-CoV-2 sequences during the first year of the pandemic,
including 👉K417N, 👉E484K and 👉N501Y, there is no evidence to suggest that these variants have been created by adding sequences of each substitution during transmission between hosts.

➡️ As only a few SARS-CoV-2 mutations were in circulation during most of 2020, it is likely
Read 5 tweets
25 Mar
➡️1) (INSACOG) The Virologist 👉Shahid Jameel explained that a double mutation 👉in key areas of the virus's spike protein can increase these risks and allow the virus to escape the immune system; making the virus 👉more contagious and in some cases.
2) Is more probable that what emerged from my research 👉SARS COV 2 ORF 8 created a double mutation in the initial lineage.
👉The ORF8 protein has the characteristic of potentiating the virus and thus making it more virulent and infectious.
Read 13 tweets

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