Daoyu Profile picture
10 Apr, 4 tweets, 5 min read
@gdemaneuf @ParkSuAm1996 archive.is/n1RwU

archive.is/5cCa8

archive.is/N4JUA

archive.is/5xElt

archive.is/EiCQW
There are evidence that the WIV practiced making up metagenomes as early as in early 2018. See that MN611520.1. Claimed bat sample, identical virus found
@gdemaneuf @ParkSuAm1996 In maize without any bats.

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More from @Daoyu15

11 Apr
archive.is/HreZB
Shi webinar: RaTG13 binding to both human and bat ACE2 is “weak”. Image
ImageImage
Read 13 tweets
10 Apr
@sriver32500052 @TheSeeker268 The ACE2 found in the SRA is Ra4331. This is actually 1AA different and had terrible binding efficiency. drive.google.com/file/d/1__C2Ug…
@sriver32500052 @TheSeeker268 It is not 9479. Also Ra9479 bind SARS-CoV most efficiently with very high binding affinity, comparable to human ACE2, binding to everything else is much reduced. Adaptation to Ra9479 will quickly wipe the D501 out and make it a T (as SARS-CoV) or a A (as in SHC014-CoV).
@sriver32500052 @TheSeeker268 blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=…
It turned out that the ACE2 from the RaTG13 SRA (Ra4331) is actually 1AA different from the one used here (Ra9479), which confers a catastrophic effect on binding. Ra9479 is unsolicited as there exist no support for it's existence anywhere in SRA. Image
Read 10 tweets
10 Apr
vixra.org/abs/2010.0164

archive.is/avFbS
The Spike associated with these sequences aren’t even functional. The 5’-UTR for RacCS203 got inverted(all at the dame site for all 5 sequences). Non-random error. Only happen with synthetic DNA.
arxiv.org/abs/2012.00627
The RmYN02 public SRA is a mess. Not much bat there. All human rRNA 3’-ETS.
Read 4 tweets
9 Apr
Illustrating the difference between (-)ssRNA and (+)ssRNA viruses.
Read 4 tweets
5 Apr
@pathogenetics @stgoldst @medpagetoday ncbi.nlm.nih.gov/pmc/articles/P…

jvi.asm.org/content/88/12/…
Serial passage of H5N1 and H7N1 flu in ferrets creates highly pathogenic strains that not only with increased virulence in ferrets, but also in humans. They are the reason why the initial GOF memoratorium was installed in the first
@pathogenetics @stgoldst @medpagetoday The only place where a virus is attenuated in passage is in embryonated chicken eggs or in cells left at room temperature (22C). In human cells at 37C there is no attenuation, nor there are any in live ferrets or any of the humanized animal models (hACE2 transgenic mice).
Read 37 tweets

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