Daoyu Profile picture
8 Jun, 15 tweets, 10 min read
@lab_leak @uacjess CGGCGG is banned because it is an extremely powerful immune adjuvant. Feline coronavirus have CGGCGA precisely because CGGCGG is too immunogenic and will destroy the ability for it to persist in nature.
@lab_leak @uacjess CCACGGCGAGCA more precisely. Notice that this break up the palindrome within the sequence—CpG palindrome sequences are considered one of the best vaccine adjuvants known for mRNA vaccination. The CGGCGG itself is specifically patented as part of the adjuvant sequence.
@lab_leak @uacjess journals.asm.org/doi/abs/10.112…
In fact, it is so crippling that the ZAP protein, normally only able to restrict CpG-rich viral genomes, restricts SARS-CoV-2 just as efficiently as any other CoVs. The S gene expression is specifically restricted.
@lab_leak @uacjess The CGGCGGGCACGTAG sequence is an optimal ZAP binding sequence.
“Unexpectedly, ZAP-S reduced the levels of S-encoding and N-encoding viral mRNAs substantially (10-fold to 15-fold) more efficiently than those of the full-length SARS-CoV-2 RNAs (2-fold to 3-fold; Fig. 6C).”
@lab_leak @uacjess “Targeting of S-encoding mRNAs agrees with the reduction of S protein expression observed in the presence of ZAP”. This clearly show why CGGCGG doublets can’t appear there—especially in the S gene.
@lab_leak @uacjess CGGCGGCCGCCG fold-like structures form when CGGCGG is converted into dsRNA by the RdRp, eliminating the need for a palindrome.
@lab_leak @uacjess CGGCGG and it’s antisense sequence when paired (in a hairpin or in dsRNA), is a precise sequence asked for as an adjuvant for mRNA vaccination
@lab_leak @uacjess It is possible that the initial CCT is not CCT but CCG. SARS-CoV-2 have an extreme excess of G->T transversions during early transmission. CGGCGA is not asked for as a part of the adjuvant sequence, indicating that it is not sufficiently immunogenic to be used or studied as one.
@lab_leak @uacjess CCGCGGCGGGCACGTAG will be one of the most powerful mRNA vaccine adjuvant sequences possible— especially if converted into dsRNA by the RdRp.
@lab_leak @uacjess sciencedirect.com/science/articl…
That also include the PRRA it expresses—a powerful cross-reactive epitope even against bacteria!
@lab_leak @uacjess pnas.org/content/117/41…
It is actually a superantigen—a powerful hyperinflammation promoter site that causes an extremely strong immune response. Perfect for one-off infection with perfect sterilizing immunity. Not seen in SARS-CoV.
@lab_leak @uacjess The QTQTNSPRRAR itself is also an extremely potent superdominant T cell epitope.
@lab_leak @uacjess Bound by all MHCs.

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More from @Daoyu15

9 Jun
@InWuchang @amymaxmen There exist no need for any hacker to hack a scientific database about viruses (entirely unprofitable to hack and have no significance pre-outbreak whatsoever) at September 2019. The database went offline before the need to hack arises. And it have continued to be down
@InWuchang @amymaxmen Even until today. web.archive.org/web/2020012520… the database was stuck in an infinite loop and inaccessible even today. No hacking attempts are persistent over a year and a half. The database is not an “excel spreadsheet” as proclaimed by Shi in her interview. It is a 61.5MB SQL
Read 28 tweets
7 Jun
@NeBirgitta @Parsifaler The extreme specificity to the lungs at the expense of other organs (especially the intestines, which is the reservoir of CoVs in the wild) is an extremely unnatural property. Wild animals don’t typically contact through the respiratory route—contact between different wild
@NeBirgitta @Parsifaler Animals are mediated primarily through the fecal-oral route as feces are more durable than aerosols, which don’t stay for time sufficient to span between the average time between meeting between individuals of typical wild animals. WuHu-1, or D614, does not have efficient
@NeBirgitta @Parsifaler Transmission through aerosols (unlike G614). An infection that is focused on the lower respiratory tract is not really transmissive but is very severe and will kill the host rapidly. This is the polar opposite to what that is needed for persistent transmission and survival in
Read 8 tweets
1 Jun
SARS-CoV-2 Can’t infect a BAT! RpYN06 can NEVER meet that S!
We investigated the susceptibility of primary cells from Rhinolophus ferrumequinum and Myotis species, as well as of established and novel cell lines from Myotis myotis, Eptesicus serotinus, Tadarida brasiliensis and Nyctalus noctula, to SARS-CoV-2 infection. None of these cells
were sensitive to infection, not even the ones expressing detectable levels of angiotensin-converting enzyme 2 (ACE2), which serves as the viral receptor in many mammalian species. The resistance to infection was overcome by expression of human ACE2 (hACE2) in three cell lines,
Read 6 tweets
31 May
rcsb.org/3d-view/6ACD
drive.google.com/drive/folders/…
The DRNTRD(E) sequences found in SARS-CoV and many bat-CoVs are in fact, NOT furin sites. this is because the sequence is located in a highly conserved position that is on the inside of the S2 subunit, shielded behind the S1 from
the other protomer. This site is not only completely furin resistant (both end was topologically anchored behind the other S1 and therefore remain bound to S2 and inaccessible to furin binding) but is in fact, located inside a trypsin-resistant zone of the S protein.
the PDB entry rcsb.org/3d-view/6ACD is the SARS-CoV S treated with low temperature and Trypsin. despite having both S1-S2 and S2' cleaved by trypsin, the RNTR sequence remained intact and uncleaved.
rcsb.org/3d-view/7CN8 is a homologous S with the sequence KNTQ from GX-S.
Read 17 tweets
31 May
This is not correct. Cell penetrating peptides AKA peptides with a lot of positive charges, are extremely hard to secrete because they will stick to the membranes and go back into the cells pubmed.ncbi.nlm.nih.gov/24928321/ even if it was prevented from being cleaved by furin.
This is in fact one of the reasons why the SARS-CoV-2 virion carry much less Spike than SARS-CoV per virion, especially if a pseudotype system is used and the S was D614.
Consecutive positive charges in cells are associated with NLS sequences of intracellular proteins. Not exactly what you see on Spike proteins. Some of these basic sites are associated with VERO E6 passage and is deleted in live hosts.
Read 6 tweets
30 May

Viral recombination does not respect the reading frames of the target RNA. The nucleotide sequence is not present in any other RNA that the virus could encounter. It is absent in mammals or viruses, particularly the genomes and transcriptomes of bat,
This mean that there exist no source of the insert anywhere in plausible hosts and plausible intermediate hosts for SARS-CoV-2, and the only RNA that it could encounter for sufficient terminal homology that allow it’s insertion by recombination in a mammalian cell will be the
Read 4 tweets

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