Daoyu Profile picture
9 Jun, 28 tweets, 20 min read
@InWuchang @amymaxmen There exist no need for any hacker to hack a scientific database about viruses (entirely unprofitable to hack and have no significance pre-outbreak whatsoever) at September 2019. The database went offline before the need to hack arises. And it have continued to be down
@InWuchang @amymaxmen Even until today. web.archive.org/web/2020012520… the database was stuck in an infinite loop and inaccessible even today. No hacking attempts are persistent over a year and a half. The database is not an “excel spreadsheet” as proclaimed by Shi in her interview. It is a 61.5MB SQL
@InWuchang @amymaxmen Database that have been erased blank before the outbreak.
@InWuchang @amymaxmen The SQL database have only been added in 06/2019 and have then be taken down at 09/2019. It is neither an “excel spreadsheet” nor is 10 year old.
@InWuchang @amymaxmen You know that a mere 3 hour visit isn’t sufficient to allow every member of the “team” to even enter the BSL-4 and BSL-3 labs, let alone actually properly examining the records on all machines—you didn’t even ask for the database!
@InWuchang @amymaxmen Shi's endorsement that it is "an excel spreadsheet" when it really WASN't.
@InWuchang @amymaxmen ncbi.nlm.nih.gov/pmc/articles/P…
They can easily ressurect any viruses out of dead sequences.
pubmed.ncbi.nlm.nih.gov/19036930/
They can also make up any consensus sequence and resurrect them into live replicating viruses.
@InWuchang @amymaxmen Minks across China were 100% negative.
@InWuchang @amymaxmen Genetic manipulation todat NEVER leave any signatures unless the researchers want to. nature.com/articles/s4158… sciencedirect.com/science/articl…
If they didn't disclose the location and identity of their markers, nobody could distinguish them from evolutionary changes in any way.
@InWuchang @amymaxmen threadreaderapp.com/thread/1402338…
A CGGCGG dimer in this location is an extremely potent attenuating agent and an extremely powerful vaccine adjuvant. the QTQTNSPRRAR sequence is an extremely powerful and promiscuous T effector epitope. it is precisely due to these properties that CGG-CGG
@InWuchang @amymaxmen on a QTQTNSPRRARS can't be found in nature--especially on the S genes of Coronaviruses.
@InWuchang @amymaxmen It is also not suspicious to find a market near any outbreak because on average of over 10000 food related markets can be found in each major city. it is just that the crowded environment is suitable for superspreading events. biorxiv.org/content/10.110… the Huanan Seafood Market
@InWuchang @amymaxmen have been entirely ruled out--while the initial outbreak have been linked to the WIBP dormitories--just as expected when the lab workers there get off shift and come home each night, where they takes off their PPE and become capable of transmission of a lab-acquired infection.
@InWuchang @amymaxmen ncbi.nlm.nih.gov/pmc/articles/P…
journals.plos.org/plospathogens/…
Unfortunately, at least 4 disclosed full sequences are alive at WIV, not the 3 (WIV1, WIV16, Rs4874). there exist RsSHC014 as a live infectious clone and at least 2 Spikes (WIV1-Rs4231S and WIV1-Rs7327S) have been rescued in rWIV1
@InWuchang @amymaxmen clones. Your identification of only one minor example of SARS-CoV leak but there is at least one major leak with 200+ quarantines in Beijing--not exactly "small and contained".
armscontrolcenter.org/wp-content/upl…
researchgate.net/publication/34…
@InWuchang @amymaxmen vixra.org/pdf/2010.0164v… See the issue with RmYN02. It had no bats in it!
@InWuchang @amymaxmen
Why the lack of pervalence for SARS-CoV-2 in animals are important: in SARS-CoV and MERS-CoV, 85% of the suceptible animals were positive near the origin of the outbreak.
@InWuchang @amymaxmen arxiv.org/abs/2104.01533
Neither the WIV-originated HKU5 in SRR5885860 nor the HKU4 in PRJNA602160 have been published anywhere before. The WIV made up MN611520.1 as the same sequence is found in SRR7896912 without any bats.

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More from @Daoyu15

8 Jun
@lab_leak @uacjess CGGCGG is banned because it is an extremely powerful immune adjuvant. Feline coronavirus have CGGCGA precisely because CGGCGG is too immunogenic and will destroy the ability for it to persist in nature.
@lab_leak @uacjess CCACGGCGAGCA more precisely. Notice that this break up the palindrome within the sequence—CpG palindrome sequences are considered one of the best vaccine adjuvants known for mRNA vaccination. The CGGCGG itself is specifically patented as part of the adjuvant sequence.
@lab_leak @uacjess journals.asm.org/doi/abs/10.112…
In fact, it is so crippling that the ZAP protein, normally only able to restrict CpG-rich viral genomes, restricts SARS-CoV-2 just as efficiently as any other CoVs. The S gene expression is specifically restricted.
Read 15 tweets
7 Jun
@NeBirgitta @Parsifaler The extreme specificity to the lungs at the expense of other organs (especially the intestines, which is the reservoir of CoVs in the wild) is an extremely unnatural property. Wild animals don’t typically contact through the respiratory route—contact between different wild
@NeBirgitta @Parsifaler Animals are mediated primarily through the fecal-oral route as feces are more durable than aerosols, which don’t stay for time sufficient to span between the average time between meeting between individuals of typical wild animals. WuHu-1, or D614, does not have efficient
@NeBirgitta @Parsifaler Transmission through aerosols (unlike G614). An infection that is focused on the lower respiratory tract is not really transmissive but is very severe and will kill the host rapidly. This is the polar opposite to what that is needed for persistent transmission and survival in
Read 8 tweets
1 Jun
SARS-CoV-2 Can’t infect a BAT! RpYN06 can NEVER meet that S!
We investigated the susceptibility of primary cells from Rhinolophus ferrumequinum and Myotis species, as well as of established and novel cell lines from Myotis myotis, Eptesicus serotinus, Tadarida brasiliensis and Nyctalus noctula, to SARS-CoV-2 infection. None of these cells
were sensitive to infection, not even the ones expressing detectable levels of angiotensin-converting enzyme 2 (ACE2), which serves as the viral receptor in many mammalian species. The resistance to infection was overcome by expression of human ACE2 (hACE2) in three cell lines,
Read 6 tweets
31 May
rcsb.org/3d-view/6ACD
drive.google.com/drive/folders/…
The DRNTRD(E) sequences found in SARS-CoV and many bat-CoVs are in fact, NOT furin sites. this is because the sequence is located in a highly conserved position that is on the inside of the S2 subunit, shielded behind the S1 from
the other protomer. This site is not only completely furin resistant (both end was topologically anchored behind the other S1 and therefore remain bound to S2 and inaccessible to furin binding) but is in fact, located inside a trypsin-resistant zone of the S protein.
the PDB entry rcsb.org/3d-view/6ACD is the SARS-CoV S treated with low temperature and Trypsin. despite having both S1-S2 and S2' cleaved by trypsin, the RNTR sequence remained intact and uncleaved.
rcsb.org/3d-view/7CN8 is a homologous S with the sequence KNTQ from GX-S.
Read 17 tweets
31 May
This is not correct. Cell penetrating peptides AKA peptides with a lot of positive charges, are extremely hard to secrete because they will stick to the membranes and go back into the cells pubmed.ncbi.nlm.nih.gov/24928321/ even if it was prevented from being cleaved by furin.
This is in fact one of the reasons why the SARS-CoV-2 virion carry much less Spike than SARS-CoV per virion, especially if a pseudotype system is used and the S was D614.
Consecutive positive charges in cells are associated with NLS sequences of intracellular proteins. Not exactly what you see on Spike proteins. Some of these basic sites are associated with VERO E6 passage and is deleted in live hosts.
Read 6 tweets
30 May

Viral recombination does not respect the reading frames of the target RNA. The nucleotide sequence is not present in any other RNA that the virus could encounter. It is absent in mammals or viruses, particularly the genomes and transcriptomes of bat,
This mean that there exist no source of the insert anywhere in plausible hosts and plausible intermediate hosts for SARS-CoV-2, and the only RNA that it could encounter for sufficient terminal homology that allow it’s insertion by recombination in a mammalian cell will be the
Read 4 tweets

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