🧲The 👉PEG nano-capsules, containing the mRNA, are magnetized due to the use of the SPION technology, during the in-vitro culture phase of the nano-capsules.👇
➡️🧲Synthesis and characterization of SPIONs.👇

1)Superparamagnetic iron oxide nanoparticles (SPIONs) were prepared by alkaline co-
precipitation of ferric and ferrous chlorides in aqueous solution in the presence of trisodium
citrate as an electrostatic stabilizer.
2) 1 Oxygen was removed from the solution by flowing N2 gas
through the reaction medium in a closed system during the synthesis reaction.
The resulting
black precipitates were collected and removed from solution by applying an 🧲external magnet and
3) then washed four times, first with de-ionized (DI) water, twice with ethanol, and finally with DI
water to remove excess ions and salts from the suspension.
The washed precipitate was then
dispersed in 20 ml DI water.
Zetasizer Nano ZS (Malvern
4) Instruments Ltd., UK) was used to
determine the hydrodynamic diameter and zeta potential of these particles in suspension, while
TEM (CM20 Philips) was used to confirm the size and morphology of dry particles. X-ray
powder diffraction (XRD) (Philips
5) 1140 PW diffractometer with nickel-filtered Cu Kα radiation
(λ = 1.5405 A°) was used to determine the crystallinity and phase of iron oxide particles.
Magnetic saturation was measured using a vibrating sample magnetometer (VSM, RIKEN
6) DENSHI) under a magnetic field of up to 15 kOe at the room temperature.
X-Ray diffraction pattern of the prepared SPIONs was identical to the standard X-Ray
diffraction pattern of pure magnetite JCPDS (Joint Committee on Powder Diffraction Standards)
7) Card No. 01-072-6170. There was a low broadening of the diffraction peaks, indicating a
decrease in the crystallization of magnetite grains.
This may be due to the preparation of
magnetite nanoparticles in the presence of trisodium citrate that
8) could significantly diminish the
crystal growth of the magnetite grains, or the generation of bubbles due to high stirring during
the synthesis process, causing oxidation to some parts of the magnetite nanoparticles.👇

pubs.acs.org/doi/10.1021/la…

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More from @BidoliNicola

8 Jun
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The biological warfare program consists of three levels:

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