This thread is to shed a bit of light on the mysteries of how much spike protein is being made, where it is going and how is it going there. Sadly our captured regulatory agencies did not insist the mfr do so
1/ I start by estimating how much spike protein is being created by the mRNA vaccines
2/We know there is 30 microgram for Pfizer
3/Molecular weight is estimated at > 1.25 x10^6 gm/mole. (~330 gm per mole of nucleotide)
This comes out to
30 x 10^-6 grams/
1.25 x 10^6 gm/mole
=24 x 10^-12 moles
6 x 10^23 molecules/mole x 24 x 10^-12 moles =14.4x 10^12 molecules mRNA (14.4trillion)
4/14.4 trillion spike protein if 1 mRNA produces 1 spike protein
5/So basically 30 micrograms of mRNA will theoretically produce about 14,400 trillion individual spike proteins (if 100% efficient)
6/Now we ask this. How many cells are transfected by the vaccines. In other words, how many mRNA molecules are in each LNP
7/Since we can estimate how many mRNA molecules there are (14.4 trillion for Pfizer), we must estimate how many LNP’s
8/First we must estimate the size of LNP
“Together with the mRNA, these components form particles of about 60–100 nm in size by using a rapid mixing production technique (Evers et al., 2018).”
9/So lets call it 80 nm and assume a spherical shape.
10/So LNP particles of 80 nm have a total weight of about 0.5 mg. Lipids are typically less dense than water but using the density of water we may estimate the volume of 0.5 mg as 5 x 10^17 cubic nm
11/ An individual 80 nm dia LNP particles volume is 256,000 cubic nm or 2.6 x 10^5 cubic nm/particle
5 x 10^17 volume /2.6 x 10^5 volume/particle =
2 x 10 ^12 LNP particles
And we already estimated
14,400 x 10^12 spike 14.4 x 10^12 mRNA
10^12 is 1 trillion (big number)
12/so basically each LNP will hold about 7 mRNA molecules. And at most 2 x 10^12 cells will be transfected, although its likely multiple LNP will transfect a cell. We just have no way to estimate this
13/Current estimates of cells in human body are 37 x10^12, and we have 2 x 10^12 LNP and 14.4 x 10^ 12 mRNA that will produce up to 14,400 x 10^12 spike protein
14/Obviously these are ballpark numbers. Its a shame we don’t have precise numbers on these questions and so many others such where does the mRNA end up and how long before its degraded
15/ Its simply incredible to me that they were not required to do a biodistribution study on primates yo find out where the mRNA goes to after its injected.
16/Pfizer used a luciferase mRNA but only tracked the lipids in the LNP. Moderna used a study on a different mRNA and lipids . Both of them used mice
16a/Using the Moderna study we see mRNA is widely distributed but in small amounts. However, if we remember that mRNA produces roughly 1000 times its weight in protein then maybe its not as insignificant as we think
17/The other thing we want to know is where does the spike protein end up. We know that once produced in the cell its presented on the cell surface (and some is chopped up and presented on MHC-I or MhC-2 molecules depending on the cell type)
18/We know that the cells transfected and which produce spike protein are destroyed by the immune cells or the cells undergo apoptosis or necrosis, and the idea is that the protein is completely destroyed
19/ yet we also have seen studies showing the S1 protein in the plasma of nurses after vaccination for up to 28 days after vaccination, and also the entire spike protein for a shorter period
21/ Furthermore Dr Patterson has discovered that S1 persists in non-classical monocytes for up to 15 months after infection and is seeing the same in post-vaccination patients.
22/These monocytes repair blood vessel wall and crawl over the blood vessel and may be causing inflammation which cause long haul symptoms
23/The question for all that is how an IM injection can lead to LNP/mRNA or spike proteins into the circulatory system. Some Doctors say its not possible, they are too big but don’t mention Transcytosis
In 1967 through the late 1980s, Israel helped the Muslim Brotherhood establish itself in the occupied territories. It assisted Ahmed Yassin, in creating Hamas, betting that its Islamist character would weaken the PLOordoabchao.ca/articles/isrea…
In 1965, Yassin had been arrested by
Egyptian intelligence, but after 1967, when Israel took control of the West Bank and Gaza, Yassin was freed.
In 1973, under approval from Shin Bet, Yassin founded the Islamic Center, and began to establish effective control over hundreds of mosques.
For over a decade, Netanyahu has lent a hand, in various ways, to the growing military and political power of Hamas. Netanyahu is the one who turned Hamas from a terror organization with few resources into a semi-state body.
Releasing Palestinian prisoners, allowing cash transfers, as the Qatari envoy comes and goes to Gaza as he pleases, agreeing to the import of a broad array of goods,
construction materials in particular, with the knowledge that much of the material will be designated for terrorism and not for building civilian infrastructure,
I will admit, this did catch my eye (from Pfizers response)
“...when a full virus does not contain any known gain of function mutations, such virus may be engineered to enable the assessment of antiviral activity in cells....”
This is related to Paxlovid and according to Pfizer is required for all anti-virals
Of course, nobody in their right mind would release such viruses into the wild without first testing on animals and no in-vivo testing is being conducted
So here is how the Hegelian Dialectic Game is played. The ELite fund both the Official Narrative and the Controlled Opposition Counter Narrative
The Official and Counter Narrative are a False Dichotomy. For example, you are allowed to consider Origins of Sars-Cov-2 as Natural or Accidental Lab Release from Wuhan. But not to entertain the idea of intentional release, by non-China actors (eg: by WEF-USG collaborators)
Anyone attacking (arguing against) the Official Narrative is a Conspiracy Theorist or Foreign Disinformation Agent and Censored (suspended, shadow banned, etc).
Many of the Twitter banished went over to Substack and built big paid followings. Twitter looking at ways to monetize and according to Elon might be interested in buying Substack
Many of the banished that were on Substack are now being returned to Twitter (and still on Substack) as Substack makes it easier to post substack articles direct to your Twitter account. Substack has also adopted a Twitter like check mark system for those with lots of subscribers
The synergy between Substack and Twitter is obvious. Twitter gets to use Substacks payment infrastructure and model while Twitter brings its algorithms used to amplify or limit reach and censor
So I woke up to the news of the subway shooting in NY. Shooter still on the loose. Looked for pictures of the shooter since surely they have them and it would help to find him. Nothing
Then I read this. “Surveillance video from within the station may not be available. A preliminary review indicates there was some sort of malfunction with the camera system at the station, Adams told WCBS Radio 880 AM”
You can not make this stuff up. Thats like no video cameras were operating at Logan yo capture the hijackers going through on 9/11