Thread by @TheSeeker268

@Ayjchan @theintercept @MaraHvistendahl @fastlerner WIV budget justification (Shi Zhengli, Peng Zhou, Ben Hu):

i) RNA extractions for 1,000 bats per year.
ii) RT-PCR assays on 2,000 samples per year.
iii) DNA sequencing on 1,500 samples per year.

@Ayjchan @theintercept @MaraHvistendahl @fastlerner WIV budget justification (Lili Ren, Li Guo):

i) RNA extractions on 1,000 samples per year.
ii) RT-PCR assays on 1,000 samples per year.
ii) DNA sequencing on 3,200 samples per year.

Where is the data?

@Ayjchan @theintercept @MaraHvistendahl @fastlerner "We have developed primary cell lines and transformed cell lines from 9 bat species using kidney, spleen, heart, brain and intestine. We have used these for virus isolation, infection assays and receptor molecule gene cloning." cc @franciscodeasis

@Ayjchan @theintercept @MaraHvistendahl @fastlerner @franciscodeasis "We will isolate bat-CoVs using Vero E6 cell (susceptible SARS-CoV and MERS-CoV) and primary or transformed bat cell lines that we have developed from Myotis davidii, Rhinolophus sinicus, Myotis chinensis, Rousettus leschenaultia and other bats of China."

@Ayjchan @theintercept @MaraHvistendahl @fastlerner @franciscodeasis "In the current [2019] proposal...effectively
identify new strains and CoV lineages needed to support experimental infection studies and risk assessment."

@Ayjchan @theintercept @MaraHvistendahl @fastlerner @franciscodeasis "Of the expected 100-200 novel SARSr-CoV strains, we will down-select to prioritize for further characterization based on S genes that are: i) different from SHC014, WIV1, SARS-CoV with diversity ranges of 10-25%;" 🤔