1) "Construction of recombinant viruses: Recombinant viruses with the S gene of the novel bat SARSr-CoVs and the backbone of the infectious clone of SARSr-CoV WIV1 were constructed using the reverse genetic system described previously.
2)The fragments E and F were re-amplified with primer pairs...The fragment Es and Fs were both digested with BglI (NEB) and BsaI (NEB). The Rs4231 S gene was digested with BsmBI. The Rs7327 S gene was digested with BsaI.
3)The other fragments and bacterial artificial chromosome (BAC) were prepared as described previously. Then the two prepared spike DNA fragments were separately inserted into BAC with Es, Fs, and other fragments.
4)The correct infectious BAC clones were screened. The chimeric viruses1 were rescued as described previously."

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