Daoyu Profile picture
14 Jan, 9 tweets, 7 min read
@AngloScot2 @WisdomRebel In addition to the extremely high stability of PRRA in CaLu-3 and it's lack of stability in-vivo, some of the earliest patients in Wuhan harbored, by digital PCR and nanopore sequencing, large deletions across the QTQTN and SPRRARS archive.is/n1jS5 that are specific to
@AngloScot2 @WisdomRebel VERO E6 cell cultured SARS-CoV-2 strains--indicating that some of the quasispecies from the original cell culture were held over into the very first patients of SARS-CoV-2, strongly supporting the hypothesis that the PRRARS sequence being the result of composite selection
@AngloScot2 @WisdomRebel pressure generated from serial passage in CaLu-3 with stock preparation and storage in VERO E6 between passages, standard laboratory practices when it comes to the serial cell passage of viruses, which may also be employed if a transmissible vaccine (respiratory transmission with
@AngloScot2 @WisdomRebel no pathogenesis in aged WT BalB/C mice). @PingThread Unroll
@AngloScot2 @WisdomRebel no pathogenesis in aged WT BalB/C mice). @threadreaderapp Unroll
@AngloScot2 @WisdomRebel no pathogenesis in aged WT BalB/C mice).

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More from @Daoyu15

14 Jan
@scotub Composite selection pressure in the form of serial passage in CaLu-3 and storage in VERO E6 leads to the specific favoring of PRRAR over RRRAR and RRRARR. Especially the storage part since the only cell line which stocks of CoVs are stored in is VERO E6 and not any other cell
@scotub lines incl. TMPRSS2+ cell lines of VERO E6. This cell line is completely intolerant to an RRRAR strain, but can store PRRAR past passage 2. inoculation of CaLu-3 when passage is resumed, quickly purifies away the deleted genomes.
@scotub ncbi.nlm.nih.gov/pmc/articles/P…
In fact, in clinical samples in Wuhan, the VERO E6-specific deletion variants with the NSPRRAR,NSPRRARSVA and QTQTN deleted, are readily detectable with digital PCR--but not the delPRRA as in the ancestral Sarbecovirus Spike.
Read 8 tweets
13 Jan
rcsb.org/3d-view/7DMQ/1
Cas13 in complex with CrRNA. The 3’ end of the CrRNA is exposed which gives interesting applications to this......
ncbi.nlm.nih.gov/pmc/articles/P…
The common RT-Cas1 and cas2 have a protospacer length of "34bp to 36bp".
Read 34 tweets
11 Jan
@factcheckdotorg archive.md/hMp7x
Unfortunately this is not true. The grant that is being mentioned were one that is already finished at the time of 2016. What that is actually important and actually contain (in the scope) viruses related to SARS-CoV-2 is the grant that is active in
@factcheckdotorg archive.md/lEKZQ the beginning of 2019 and continue to 2024. The grant is already active in 2019 since the MERS-like viruses studies in the grant is found already escaping the WIV and lurking inside HZAU.
Read 8 tweets

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