Tan CC, Eckardt KU, Ratcliffe PJ. Organ distribution of erythropoietin messenger RNA in normal and uremic rats (1991) doi.org/10.1038/ki.199… EPO mRNA was measured in the kidneys of unstimulated control animals and was detectable, at lower levels, in the liver and lung #mEPO 💉
After exposure to hypoxia, there was a 150-fold increase in renal mEPO. Hepatic EPO mRNA was also greatly increased and accounted for 39% of the total. A small quantity of EPO mRNA was in lung. Animals subjected to subtotal nephrectomy became uremic and anemic. Let’s compare💉👇 
EPO protein is a secreted protein that is easily measured in serum by enzyme-linked immunosorbent assay. EPO stimulates red blood cell creation that can be measured using a hematocrit assay. EPO mRNA is available to model your secreted protein calling mEPO trilinkbiotech.com/products-servi…
Masks. We studied the effects of hypoxia and CoCl2 on the rate of Epo gene transcription. We showed that steady-state mEPO levels increase more than 50-fold in response to hypoxia or CoCl2, nuclear run-off experiments demonstrated only a 10-fold increase in Epo gene transcription
(1990) Erythropoietin mRNA Levels Are Governed by Both the Rate of Gene Transcription and Posttranscriptional Events. Mark A.Goldberg Christopher C.Gaut H. FranklinBunn. Hematology Division, Brigham and Women’s Hospital and Harvard Medical School, Boston doi.org/10.1182/blood.…
This mEPO is capped using a co-transcriptional capping method, which results in the naturally occuring CAP1 structure with high capping efficiency. It is polyadenylated, modified with ψUTP and „optimized for mammalian systems” #zaJob apexbt.com/molecular-biol…
The addition of ψUTP and poly(A) tail can reduce host cell immune response and enhance mRNA stability. It can simulate the function of a fully processed mature mRNA. It can reprogramming mEPO, i.e mRNA for non-integrating generation of iPSC💉(PCR „test”😉) apexbt.com/ez-captm-epo-m…
There has been described in January 2014 how regular blood cells could be turned into pluripotent stem cells. Obokata, Sasai, Niwa et al. Retraction Note: Bidirectional developmental potential in reprogrammed cells with acquired pluripotency doi.org/10.1038/nature… End of Health
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