Updates on plans for outy sequencing #nanoporeconf
Outy sequencing accuracy #nanoporeconf
Detection of very low frequency variants with outy sequencing #nanoporeconf
Also improves modified basecalling #nanoporeconf

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More from @Amandatron89

Oct 18
Recently I convinced the institute to let me buy pizza for the campus once a month, not as a moral boosting strategy, but as bait.

Today with the postdoc committee, I hosted the first Pizza Solves Everything event
Rather than having seminars on polished, finished projects, I invited people who were stuck and struggling to overcome a research problem to come and do a short presentation explaining their struggles. Importantly no one would be questioned in front of an audience.
Following the four 5-10 minute presentations everyone got free pizza...

Well, almost everyone. So many people came that 96 slices of 18-inch pizza were insufficient.
Read 9 tweets
Oct 11, 2021
Lilo: A pipeline to stitch together tiled amplicons from Nanopore sequencing data.

github.com/amandawarr/Lilo
But Amanda, that pipeline already exists.

Yes, I know that, me, but the genomes I spend my days arguing with have specific features that make them incompatible with the @NetworkArtic pipeline.
The artic pipeline is based on aligning reads to a reference and polishing the reference to look like the reads. That works great, but...
Read 15 tweets

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