Giulia Casal Profile picture
Feb 6, 2023 14 tweets 8 min read Read on X
🧵🪡A thread on our paper out in today’s @Dev_Cell issue, featuring one of my images on the cover 🤩 Image
You might’ve heard about the #BBB but have you heard about the BNB (Blood Nerve Barrier)? #pns #bloodnervebarrier Image
We showed that endoneurial blood vessels (EndoBVs) are surrounded by pericytes (alphaSMA, orange) 🤗 @ImarisSoftware
And another component of the vascular unit being tactocytes (green, with characteristic rER) in close contact with the basement membrane of EndoBVs Image
The last cell of the vascular unit of the BNB are macrophages (Iba1 or F4/80, blue) 🐭 @ImarisSoftware
We found that the VU is conserved in rat 🐀 and human 👩🏽‍🤝‍👨🏼samples. Human samples shown here: beautiful #vEM z slice from @EM__Ian with characteristic endothelial cell/#pericyte peg-socket interactions & @ImarisSoftware of tactocytes and macrophages along EndoBVs Image
Next, we looked at how the BNB is conferred and showed that EndoBVs have specialised tight junctions and low levels of transcytosis. ImageImage
We then used our P0-RafTR model (previously published @NeuroCellPress) where we could “open” the BNB in a regulated fasion. Here, i.v Evans Blue 🔵 Image
And we found that opening the BNB is by strongly increased transcytosis (plvap), while tight junctions (here #claudin5) were maintained. Image
Interestingly, Liza found that #Macrophages were the only cells taking up tracers (here HRP) and that they move away from the BNB in the P0-RafTR model. In short, acting as a secondary barrier mechanism. Image
Finally, we looked at the therapeutic potential in collaboration with @ionispharma and showed increased antisense oligos (ASOs) inside endoneurial endothelial cells & the endoneurium when we open the BNB as well as significantly increased target gene downregulation. ImageImage
So much more in the paper! Check it out and tell us what you think!
Most importantly, thank you to everyone involved! Especially Liza, @EM__Ian @JemimaBurden @AnnelaureCattin & many more not on Twitter! The animals 🐭& staff looking after them, the @LMCB_UCL staff which keeps everything running 🌟 and our funders @CRUKresearch @wellcometrust

• • •

Missing some Tweet in this thread? You can try to force a refresh
 

Keep Current with Giulia Casal

Giulia Casal Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!

PDF

Twitter may remove this content at anytime! Save it as PDF for later use!

Try unrolling a thread yourself!

how to unroll video
  1. Follow @ThreadReaderApp to mention us!

  2. From a Twitter thread mention us with a keyword "unroll"
@threadreaderapp unroll

Practice here first or read more on our help page!

Did Thread Reader help you today?

Support us! We are indie developers!


This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3/month or $30/year) and get exclusive features!

Become Premium

Don't want to be a Premium member but still want to support us?

Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal

Or Donate anonymously using crypto!

Ethereum

0xfe58350B80634f60Fa6Dc149a72b4DFbc17D341E copy

Bitcoin

3ATGMxNzCUFzxpMCHL5sWSt4DVtS8UqXpi copy

Thank you for your support!

Follow Us!

:(