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Nechama Wieder (@NechamaWieder) #GRD23 starts by reminding us of how critical 5'UTRs are.
Start codons within the 5'UTRs (uAUGs) can decrease translation of the downstream coding sequence.
Start-stop: "minimum ORF" impedes ribosome scanning without peptide production of uORFs.
Start codons within the 5'UTRs (uAUGs) can decrease translation of the downstream coding sequence.
Start-stop: "minimum ORF" impedes ribosome scanning without peptide production of uORFs.
.@NechamaWieder #GRD23: Start-stop contribute to ribosome pausing and decreasing downstream translation.
~5% of all MANE Select transcripts have these start-stops and most of them only have one.
~5% of all MANE Select transcripts have these start-stops and most of them only have one.
.@NechamaWieder #GRD23: Start-stops are enriched in genes intolerant to LoF (via LOEUF deciles)... but those genes are also longer and have different GC content.
Used a shuffling algorithm to determine observed/expected per hexamer to show that the start-stops are enriched.
Used a shuffling algorithm to determine observed/expected per hexamer to show that the start-stops are enriched.
.@NechamaWieder #GRD23: Start-stop enrichment does seem to be primarily in the LoF intolerant genes.
Looking up start-stops in the Genomics England 100k Genomes Project. Found a few and are digging more into them.
Looking up start-stops in the Genomics England 100k Genomes Project. Found a few and are digging more into them.
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