Thread by @SurbhiG6

So excited to share our new preprint on the chromosome-scale annotated genome assembly of Triticum timopheevii, an allotetraploid wheat wild relative species. This is the first time when the G subgenome of Triticeae has been sequenced. Check it out! 1/
biorxiv.org/content/10.110…

The assembly was done using @PacBio HiFi reads and HiC capture using the @PhaseGenomics Proximo kit. Thank you @ivanliachko @kboortz18 for your support on this. All sequencing was done by the amazing team at @Novogene_Europe. Contig N50 = 42.4 Mb and scaffold N50 = 671.1 Mb 2/

The structural and functional annotation was done by the amazing team @EarlhamInst using the REAT pipeline. Thank you to David @swarbre and the lovely Sreya @sreyag_ 🙏 3/

When I started my apprenticeship in Bioinformatics during the first lockdown I never imagined I will be assembling genome sequence let alone at chromosome-scale for one that is over 9 Gb with 14 chromosomes! I don't know how I got here but this has been a huge learning curve. 4/

I cannot say enough about the amazing team at @Notts_WRC who supported me as I ventured into the unknown. The technicians Caiyun, Duncan and Stephen, I couldn't have done it without you guys.
Also my PhD students, mentors @sbzipk Ian and Julie King and Stella @stelwards 🙏🙏🙏 5/

A special thanks to @MichaelWilson_ and @leviyant for illuminating the dark paths along the way. To the amazing members of the Genome Reference Informatics Team at @sangerinstitute @geval_browser @SangerVGP @SangerToL that helped me manually curate the assembly. 6/

Just want to give a shoutout to the great folks in the Human Computer Interaction Lab @usask for the SynVisio browser . It saved my life! It was great to see all the known translocations in the T. timopheevii genome and find new ones! And so easy to use 7/ synvisio.github.io/#/

The genome assembly is being hosted @GrainGenes Thanks to @tanerzsen and Eric Yao for sorting this out at lightning speed. 🙏 8/wheat.pw.usda.gov/jb?data=/ggds/…

This work was supported by funding from @UKRI_News @BBSRC @UKFutureWheat @USDA and @UniofNottingham @UoNScience @UoNBiosciences @PlantSciNottm. We are super grateful for your continuing support in the delivery of this and other resources to the wheat research community🙏🙏🙏

@UKRI_News @BBSRC @UKFutureWheat @USDA @UniofNottingham @UoNScience @UoNBiosciences @PlantSciNottm @threadreaderapp unroll