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Aug 2 29 tweets 6 min read Read on X
I made a Black Aura*.

I coated a 3M Aura 9210+ in Derwent Academy black water soluble marker, and measured the resulting fit factor using a PortaCount 8020A in N99 mode. There was no meaningful impact on performance.

* It came out gray. 🧵 Three-quarter top-right view of 3M Aura 9210+ respirator sitting on a wooden desk, with a clear plastic threaded barb pipe fitting sticking out of the front panel, for connection to a PortaCount machine. The Aura's external surface is coated in Derwent Academy black marker ink, which gives it a steel gray color. The thousands of debossed oval shapes in the Aura's material are lighter than the surrounding area, giving the resulting mod a look resembling diamond plate, or maybe gray jeans.
I performed 3 sets of 3 normal breathing exercises wearing a 3M Aura 9210+ respirator, with a redonning in between each set. I then spent about 45 minutes coating the entire outer surface in Derwent Academy black marker.

And yes, it was exactly as satisfying as you'd think.
For this experiment I wanted to push the limits of modding to see if I could get the respirator to fail.

I could not!
I chose the Derwent Academy series of markers because they are vegan, and veganism is awesome.

I *mistakenly* thought they were advertised as nontoxic, but they are not. However their safety data sheet suggests they are unlikely to be toxic.

derwentart.com/a/328371SECTION 11: TOXICOLOGICAL INFORMATION. This material is unlikely to present a significant health hazard under normal conditions of handling and use. 11.1 Information on toxicological effects. Acute toxicity: Low acute toxicity. Skin corrosion/irritation: Unlikely to cause skin irritation. Serious eye damage/irritation: Unlikely to cause eye irritation. Skin sensitization data: It is not a skin sensitiser. Respiratory sensitization data: Not classified. Germ cell mutagenicity: There is no evidence of mutagenic potential. Carcinogenicity: No evidence of carcinogenicity. Reproductive toxicity:...
After coating the respirator in a layer of ink, I performed another 3 sets of 3 normal breathing exercises, with a redonning in between each set.

The result is astonishingly that there is no meaningful difference between the default and modified respirators. Line graph with title: "3M Aura 9210+ Default vs "Black Aura" Marker Mod". Subtitle: "3x3 normal breathing exercises, redonning in between. Derwent Academy black marker." The x-axis is "Normal Breathing Exercise", and the y-axis is "Fit Factor". It contains a solid blue line, "Default", and a dashed red line, "Derwent Marker". Each line is roughly horizontal, with minimal fluctuation across the 9 exercises for each. Each line contains a gap between each segment of 3 exercises, where redonning occurred. Both lines nearly o...
Here are the harmonic mean fit factors (HMFFs) for each respirator for each set of 3 exercises, plus the overall HMFF for all 9 exercises. These represent the average leakage across all exercises. Bar graph with title: "3M Aura 9210+ Default vs "Black Aura" Marker Mod". Subtitle: "3x3 normal breathing exercises, redonning in between. Derwent Academy black marker." The y-axis is "Harmonic Mean Fit Factor". There are four pairs of blue and red bars, with the groupings named: "HMFF 1-3", "HMFF 4-6", "HMFF 5-9", "HMFF All 9". The HMFFs for each set of 3 exercises for the Default respirator are: 193, 178, 243. For the Derwent Marker they are: 177, 189, 213. The 9-exercise Default HMFF is 201, and for Derwent M...
I consider these results to represent identical performance between default and modded respirator.

This sort of fluctuation in minute-to-minute fit factor is absolutely common when testing a respirator.
I used redonnings in this experiment to partially control for the impact of donning on respirator fit.

It is possible that the marker could worsen Black Aura performance, but the second donning produces a superior fit, negating the marker's measured impact.
Multiple donnings in this experiment show that the fit is consistent across donnings, for both default and modded respirator.

Given that the fit is stable, and the Black Aura did not see worse performance, we can conclude that the marker does not meaningfully impact performance.
In this detailed view, you can see that the Aura's material appears debossed, and the marker's tip glides over the sunken areas. It also does not absorb ink as paper would. I suspect the fibers are not nearly as saturated as they appear at a distance, preserving performance. Closeup photo of the seam between the front and top panels of the 3M Aura 9210+ respirator when coated in Derwent Academy black marker ink. The respirator is a medium gray color, as if it were made out of steel. The seam is slightly darker. The Aura's material contains thousands of small, sunken, oval shaped, debossed pockets, which are slightly lighter than the surrounding material. Ink seems to collect and form a border around each of these embossed pockets. The result is a respirator that somewhat resembles diamond plate.
A reminder that the amount of airflow through the respirator heavily influences the resulting score. If I breathe heavily, I can push my Aura score under 100. If I hold my breath for 60 seconds, I can push it over 1000.
For this reason, breathing rate is a semi-controlled variable in PortaCount experiments, and the resulting scores should be interpreted loosely.

What we're looking for here is an obvious difference between the two test conditions. And there isn't one!
The individual exercise scores for the default Aura ranged from 160-236. The scores for the "Black Aura" ranged from 157-226.

These sound like large fluctuations but this is just normal variation. They represent absolute differences of 0.20% and 0.19% leakage, respectively.
It's also important to note that face seal leakage dominates total leakage when wearing most respirators. This experiment is designed to answer the question: "If I get too carried away pimping out my respirator, should my friends intervene?"
In the this experiment, the HMFF of 9 normal breathing exercises was nearly identical between respirators.

The absolute difference was 0.02% leakage. This is easily explained by random variation.

They're the same! Shocking, but true!
So what can we conclude from this experiment?

Well, I'm flabbergasted. I was honestly expecting the performance to degrade. At least with this particular water-soluble marker, you can decorate as much as you want and I don't see any issue.
Unlike an OSHA style fit-test, these results will generalize to your face, since the mod is not structural and I am using my own face as a control. This is a fairly pure test of whether drawing on a respirator degrades performance enough to add meaningful leakage.
I am *medium* confident that this will generalize to pretty much every disposable respirator. I would like to see various markers tested on various models, but these results put another nail in the myth that respirators are fragile snowflakes.
However PLEASE, don't be reckless! 🙏

If you want to draw on your respi, do a Bitrex fit-test one time to verify your approach to modding is fine. Share your results with the community so we can all learn!
If you can't access Bitrex for whatever reason, I don't think you're taking a significant risk by drawing on your respi with a water-soluble marker.

However, it's not like we have a database verifying the safety of every marker. Use good judgment.
If there were any safety concerns regarding coating the outside of an Aura in velvety ink, I think this mitigates them.

Any drawing you are likely to do won't be as intense as what I just attempted! 😅
I should note, I was surprised to learn that these Derwent Academy markers contain 20% isopropyl alcohol by weight. It's flammable, so don't light your respirator on fire.

derwentart.com/a/328371Table from page 2 of the linked safety data sheet, showing hazardous ingredients in the Derwent Academy markers. The ingredients are: 1-methoxy-2-propanol (monopropylene glycol methyl ether) (<75% by weight), and propan-2-ol (isopropyl alcohol) (<20% by weight).
The study below shows 70% isopropyl alcohol degrades the electrostatic charge in respirator filters. The Derwent Academy markers contain less than 20%, possibly much less. I can only assume this is why the marker did not impact performance.

isrp.com/the-isrp-journ…Isopropyl alcohol: Fading of strap ink was the only visible change observed. As expected, both treatment conditions (1 second and 1 minute submersion) resulted in markedly increased average penetration for both respirator classes (Table III). As observed in previous studies (Martin and Moyer, 2000; Janssen et al., 2003), exposure to IPA was found to be severely degrading to N95 and P100 filters containing electret filter media. This was possibly due to changes in the density and/or spatial distribution of the electret charges on the surface of the polymer fibers by the liquid phase applicat...
Okay, on to qualitative observations.

The marker doesn't smell. It has zero odor. That's good!
However, and this is pretty disappointing: it smudges like you would not believe.

After applying the ink, I allowed it to dry for an hour, but it will still rub off the respirator as if it were freshly applied. 😬
The ink gets all over your hands and stains them. I washed my hands many, many times and it only came off slowly. I had to resort to a pumice stone to get most of it off.
Maybe other brands of marker wouldn't do this? I don't know.

Just be aware that if you put the ink in strategically poor locations, you're going to get it on your hands and face. It's probably best to avoid the edges in your designs.
Let me know if there are any respirator fashion mods you would like me to test.

Happy drawing!
Update: 19 days later, the ink *still* has not dried on the Aura! It's significantly less smudgy, but I can still rub it onto a sheet of paper.

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More from @antiviral_mktng

Sep 18
I donned a 3M Aura 9210+ respirator 20 times to measure loss of protection.

Repeated donnings appear to gradually erode protection, but the impact measured is small, and not detectable using candle smoke, only saline aerosol.

🧵 Closeup of right half of a 3M Aura 9210+ laying face down on a wooden table, with 9 black sharpie marker dots drawn on the left side of the neck strap.
I had performed another experiment (yet to be published) which required multiple donnings, and noticed that fit-tests performed using candle smoke failed to detect a gradual loss in fit that was apparent when using saline.

So I went on a side quest to explore this phenomenon.
This was also a good time to explore the impact of redonnings on respirator fit. We know that extended wear causes a loss of fit. But how much of this loss can be attibuted to the *time* spent wearing the resperator, versus the process of putting it on?

jamanetwork.com/journals/jaman…
Read 49 tweets
Aug 29
People keep arguing that "outdoor transmission is rare", so I need to explain something.

Small iterated risks are certainties.

If you keep doing a dangerous thing over and over, you will get burned.

🧵
Someone made a wild guess that the risk of outdoor transmission "is 1%".

There is no solid data to back that up, but let's start with that assumption.

Let's say on average throughout the year, 1 in 50 people are infectious.
So you're walking down the sidewalk and have fleeting outdoor interactions with people. It's really easy to pass near 50 pedestrians during your afternoon stroll.

So every day on average you pass at 1 infectious person, and experience this 1% risk.
Read 9 tweets
Aug 11
Things I miss being able to do since Long COVID:

- Running
- Cycling
- Visiting family a medium distance away, which is now a Herculean task
- Consistently sleep 8 hours
- Weightlifting
- Reading books as much as I want
- Standing upright for prolonged periods

🧵
- Sitting in a chair and not experiencing unrelenting chronic pain
- Walking into a room and knowing why I walked in there
- Eating junk food
- Drinking coffee, a brutal loss, because coffee is delicious but it makes my symptoms much worse (yes, even decaf)
- Social interactions longer than a couple hours
- Calling a friend or taking a call whenever I want to
- Playing musical instruments
- Keeping the house clean
- Working at a job to earn money to live
- Meditating, and actually getting somewhere with it
Read 11 tweets
Aug 6
I soaked a 3M Aura 9210+ in 70% isopropyl alcohol.

It destroyed the electrostatic charge and obliterated respirator performance.

This proves the PortaCount can detect dangerous mods. 🧵 3M Aura 9210+ respirator on a wooden desk. It is its original, plain, white color with no visible alterations. It has a plastic pipe fitting installed in the front for PortaCount testing.
Why would I do this? It's a positive control for my mask modding safety experiments.

In previous experiments I was able to show that graphite pencil and at least one brand of water-soluble marker *do not* impact respirator performance, even if you go buckwild with your designs.
To add confidence that the PortaCount is indeed able to detect when mods destroy the electrostatic charge, we need to... intentionally destroy the electrostatic charge! 😅
Read 27 tweets
Aug 5
IF ONLY THERE WAS SOME WAY TO OBTAIN STERILIZING IMMUNITY

3M Aura 9210+ respirator. When fit-tested and worn properly, provides essentially sterilizing immunity against airborne pathogens.
Twitter search result showing @PeterHotez has not tweeted the word "mask" since at least June 1 2024.
Hotez has not tweeted the word "mask" since September 7 2023, and has only used the word "respirator" one time EVER. Maskless indoor photo of Rebekah Jones, Peter Hotez, and Eric Feigl-Ding at a Hotez book signing event, September 16 2023.
Scientists have a moral responsibility to communicate safety strategies unambiguously to the public, and to role model use of those strategies.

Like my man Ziyad!

Read 4 tweets
Aug 1
I drew all over this 3M Aura 9210+ with a graphite pencil and measured the resulting fit factor using a PortaCount 8020A.

There was no meaningful loss in protection. 🧵 Three-quarter top-right view of a 3M Aura 9210+ respirator sitting on a wooden table. The respirator, normally white, has hundreds of gray bubbles drawn on it using a graphite pencil. The lines produced by the pencil are not sharp, due to the Aura material's texture.
I performed 6 normal breathing exercises wearing a 3M Aura 9210+ respirator, then spent about 45 minutes drawing a bunch of bubbles all over it.

For this experiment I wanted something easy to draw which would use up a lot of pencil graphite, simulating some ambitious art.
I used a 5B pencil because it's the softest pencil I have in the house. I also tried an HB pencil briefly, but the harder graphite makes drawing slightly more difficult. If you want to draw pencil art on a respirator, I would definitely recommend the softer pencil.
Read 23 tweets

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