…An unknown gene shows up on PCR from the liquid surrounding Replicon infected cells. It appears weakly after 5 generations, very faint after 10 generations, strongly after 15 generations and then almost gone after 20 generations. They don't mention in 1/2ncbi.nlm.nih.gov/pmc/articles/P…
They do not sequence this unknown gene that shows the most copies after the 15th generation and then mostly disappears. Is it a partial spike protein gene that would make their conclusion that Replicons can transfer partial genes from just the fluid around Replicon cells ? 1/3
This is Figure one from the paper above. It shows a mystery gene smaller than the spike gene show up in the first generation. There's no mystery gene in the control. The mystery gene gets fainter in the 3rd and 5th generation. (Fewer copies) 2/3