Thread by @KennyCarmody

Can you comment on SV40
promoters being LNP delivered as cytosolic DNA and the impact on the cGAS-STING pathway noted by Kwon et al.



Thread Below: 🧵 pubmed.ncbi.nlm.nih.gov/31852718/

1. SV40 promoters, lipid nanoparticles (LNPs), and the immune response. The SV40 promoter is a powerful DNA sequence that can drive gene expression, but what happens when it’s introduced into cells with LNPs?

2. First, what’s SV40? It’s a sequence from the Simian Virus 40, known to enhance gene expression. We are not sure if it’s cancer-causing but data shows clear signs that is does, SV40 promoters can potentially impact cell regulation by ramping up the activity of nearby genes.

3. Lipid nanoparticles (LNPs) are like tiny vehicles used to deliver genetic material into cells. LNPs can transport mRNA, DNA, and other molecules directly to the cell’s cytoplasm, bypassing traditional delivery barriers. This tech is widely used in gene therapy and vaccines.

4. When you deliver DNA with an SV40 promoter via LNPs, the DNA lands in the cytoplasm of cells. This can be a big deal because cells don’t expect DNA to be in the cytoplasm—only in the nucleus or mitochondria.

5. Why does this matter? It activates the cGAS-STING pathway, which is the cell’s “security system” for detecting foreign or misplaced DNA. This pathway helps our body recognize and respond to infections and other dangers.

6. When cytosolic DNA is detected, cGAS (an enzyme) triggers STING, leading to a chain reaction that releases pro-inflammatory cytokines and type I interferons. Essentially, it signals, “We’ve got an intruder!”

7. But constant activation of this pathway isn’t good. If SV40 promoter DNA fragments are persistently in the cytoplasm, they can keep cGAS-STING on high alert. This could lead to chronic inflammation and an overactive immune response and to my opinion cancer.

8. Prolonged immune activation is exhausting for the body. It can lead to immune exhaustion, where immune cells are overworked and less effective. In some cases, it may also cause severe inflammation, known as a cytokine storm.

9. There’s also a high risk of autoimmune reactions. Chronic presence of DNA in the cytoplasm could cause the immune system to mistakenly attack its own cells, as it starts to see its own DNA as a threat. This could trigger autoimmune diseases. Which it did to me with ALS & MS.

10. SV40 promoter’s gene-activating power can increase the expression of nearby genes. If these are oncogenes (genes that promote cell growth), it could push cells toward uncontrolled growth and tumor formation.
Imo that is the cause we see the rising cancer rates skyrocketing

11. On top of that, SV40 elements can sometimes interfere with tumor suppressors, like p53, a key protein in regulating cell death and preventing mutations. The risk? Higher chances of cellular mutations and cancer in the long run.

12. Now consider the dose: delivering 60 billion copies of SV40 promoter DNA via LNPs in one dose would flood cells with foreign DNA. This massive load could overwhelm immune defenses, leading to acute toxicity and severe inflammatory responses. @Kevin_McKernan

13. Short-term, we might see systemic immune responses, including inflammation, immune dysregulation, and toxicity. Long-term effects could include chronic inflammation, autoimmune diseases, & cancer.
The future is dire and we need to stand strong against this crime in humanity.

14. The takeaway? Introducing SV40 promoter DNA at such a scale is a serious stressor to the immune system. The body will struggle to handle the load, potentially leading to a cascade of harmful effects on immune function and cellular health… It’s a crime! Indeed the biggest yet

15. While LNPs are powerful tools in science, we need to understand their impacts fully especially SV40. Unchecked or large-scale use will pose significant health risks. You can’t just play with biology and don’t see any consequences. Genocide I tell you all!

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