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Briefing on Gene knockdown and knockout in today's #365DaysOfScience. These two are classical genome editing tools used to study gene function and probable effects of inhibiting the target gene. These tools find relevance in molecular genetics and drug discovery.
Gene knockout is the 'knocking out' of a gene. It is attributed by the permanent deactivation or complete elimination of gene function to evaluate the functional specificity of that gene. It is achieved by homologous recombination, endonucleases, or CRISPR/Cas9.
#365DaysOfScience
#365DaysOfScience
Using Gene knockdown the functional expression of a gene is suppressed in a flexible way. This approach is neccesiated for the genes whose KO will have a lethal effect. Working with RNA, it uses RNAi (siRNA/shRNA) leading to abortive translation/mRNA degradation
#365DaysOfScience
#365DaysOfScience
Spectroscopy allows us to spy on molecules invisible to the naked eye. Through a cuvette the spectrophotometer allows us to peer and measure concentrations with the help of Beer! 👉 bit.ly/2N4nzXE
#366DaysOfScience #biochemistry #scicomm #realtimechem
#366DaysOfScience #biochemistry #scicomm #realtimechem
The Beer Lambert law (Beer’s law) allows you to convert between concentration (c) of a dissolved thing (solute) & absorbance of light. Don't have a lot - use a NanoDrop so the UV-Vis fun don't stop! bit.ly/beerslawexplai…
#366DaysOfScience #biochemistry #scicomm #realtimechem
#366DaysOfScience #biochemistry #scicomm #realtimechem
Do you listen to your spectrograph peaks when they try to speak? Or just look at highest 1 & assume purification's done? Peakiest peak *might* tell concentration - but rest can reveal contamination! 👉 bit.ly/2NblGc2
#366DaysOfScience #biochemistry #scicomm #realtimechem
#366DaysOfScience #biochemistry #scicomm #realtimechem
The new year begins and I usher into a scientific journey where I shall be challenging myself with scientific quest and reviewing a scientific article/blog/interview/technique or facts on a daily basis. (or at least try😉) #365DaysOfScience
Let the journey begin.
Let the journey begin.
The most cited article (over 300,000 times) is the @jbiolchem paper for a technique to estimate protein concentration in a solution. The paper is "Protein measurement with the folin phenol reagent".
#365DaysOfScience
Image: @nature
#365DaysOfScience
Image: @nature
Yes, the classic Lowry's method was published in May 1951. This is a colourimetric assay which gives an estimation of the amount of protein based on a standard curve obtained from a protein of known concentration (usually BSA).
#365DaysOfScience
#365DaysOfScience
After a jam-packed 2 days at #SciOut18🌪(thx @ASBMB & @rockedu_!) it was time to reflect! 🤔 Reflection is so important, but it can be hard to find ⏰ to really just think! 💭 Some of my thoughts 👉 instagram.com/p/BokNO_NhihF/
#365DaysOfScience #biochemistry #scicomm #sciout #science
#365DaysOfScience #biochemistry #scicomm #sciout #science
What’s inside? 🧐 It’s FORMAMIDE! 🤗 Formally removing RNA’s form with formamide! 🤓 FORMAMIDE in loading buffer breaks up & replaces the bonds between bases holding RNA's shape together 👇
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#365DaysOfScience #biochemistry #scicomm #lifeinthelab #science
instagram.com/p/BomwR32B4MX/
#365DaysOfScience #biochemistry #scicomm #lifeinthelab #science
Bring on the heat! 🔥 To help denaturants get to the huddling hydrophobic bases, we add heat ♨️ 👉 gives molecules more energy so they can “pull apart” more easily ⏩ easier access 👍 More 👇
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#365DaysOfScience #biochemistry #scicomm #lifeinthelab #science
instagram.com/p/Boo2yazBDDi/
#365DaysOfScience #biochemistry #scicomm #lifeinthelab #science
