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PAPER OUT!! 🥳😁
Let me share a #tweetorial
about how a nerd moment 🤓when @foad_rouhani & I were PhD students >10yrs ago (!)
has (finally) arrived @NatureGenet
with implications in using #hiPSCs in clinical studies & research modelling
1/
nature.com/articles/s4158…
Let me share a #tweetorial
about how a nerd moment 🤓when @foad_rouhani & I were PhD students >10yrs ago (!)
has (finally) arrived @NatureGenet
with implications in using #hiPSCs in clinical studies & research modelling
1/
nature.com/articles/s4158…
A 22 yr old patient provided us with 2 skin fibroblast-derived IPSCs (F-hiPSCs) and 2 blood-derived (B-hiPSCs).
🤓: Do they differ genetically if they have differing origins?
Yep. The mutational load in F-hiPSCs was way more than in B-hiPSCs. Largely due to UV.
2/
🤓: Do they differ genetically if they have differing origins?
Yep. The mutational load in F-hiPSCs was way more than in B-hiPSCs. Largely due to UV.
2/
So what’s the prevalence of UV damage in F-hiPSCs in general? Is it seen in well-known F-hiPSC repositories?
Yep. 72% of F-hiPSCs in the largest UK repository HipSci have evidence of UV damage.
And yes we’re sure it’s UV – lots of CC>TT, transcriptional strand bias.
3/
Yep. 72% of F-hiPSCs in the largest UK repository HipSci have evidence of UV damage.
And yes we’re sure it’s UV – lots of CC>TT, transcriptional strand bias.
3/
