Discover and read the best of Twitter Threads about #MolecularMicrobiologists
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1/5
African S. Typhimurium ST313 has 23 Lineage 2-specific pseudogenes, discovered by @DrRobKingsley in 2009, inc ybjZ.
But what is function?
In collab with Jared Honeycutt @monack_lab, we've discovered that MacB (YbjZ) influences pathogenesis of ST313
journals.plos.org/plospathogens/…
African S. Typhimurium ST313 has 23 Lineage 2-specific pseudogenes, discovered by @DrRobKingsley in 2009, inc ybjZ.
But what is function?
In collab with Jared Honeycutt @monack_lab, we've discovered that MacB (YbjZ) influences pathogenesis of ST313
journals.plos.org/plospathogens/…
2/5
Genomes of 285 S. Typhimurium isolates revealed lineage-specific SNPs = macA & macB are pseudogenes
Suggesting that MacAB-TolC efflux pump is functional in S. Typhimurium ST19, but defective in ST313 Lineages 1 & 2
Genomes of 285 S. Typhimurium isolates revealed lineage-specific SNPs = macA & macB are pseudogenes
Suggesting that MacAB-TolC efflux pump is functional in S. Typhimurium ST19, but defective in ST313 Lineages 1 & 2
3/5
Beautiful expts designed by Jared Honeycutt & @monack_lab show MacA variant & truncated MacB protein made ST19 sensitive to C18G antimicrobial peptide
& the ST313 macAB SNPs reduced intra-macrophage replication & fitness in murine GI tract
See @monack_lab tweet for details
Beautiful expts designed by Jared Honeycutt & @monack_lab show MacA variant & truncated MacB protein made ST19 sensitive to C18G antimicrobial peptide
& the ST313 macAB SNPs reduced intra-macrophage replication & fitness in murine GI tract
See @monack_lab tweet for details
