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We are so excited to present our functional #lineagetracing tool, CaTCH, developed in the @obenaufa lab @IMPvienna published @naturebiotech. Have you ever wondered if a phenotype is pre-existing in a cell or whether it is acquired during selection? Then👇might be your tool!1/14
CaTCH - short for “CRISPRa Tracing of Clones in Heterogeneous cell populations” - combines precise mapping of the lineage history of millions of cells with the ability to isolate any given clone alive from a complex population based on genetic #barcodes. 2/14
In a selection experiment, CaTCH allows to first identify clones with a certain phenotype and subsequently “travel back in time” to enable the retrospective isolation and analysis of founding clones from heterogeneous cell populations prior to their evolutionary selection. 3/14
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