Discover and read the best of Twitter Threads about #RaTg13

Most recents (13)

Ce qui s’est réellement passé dans le laboratoire de #Wuhan quelques semaines avant que la #Covid n’éclate,De nouvelles preuves tirées de dossiers confidentiels révèlent que des scientifiques chinois ont fusionné des agents pathogènes mortels peu avant la pandémie. Image
Des scientifiques de #Wuhan travaillant aux côtés de l’armée chinoise ont combiné les #coronavirus les plus mortels du monde pour créer un nouveau #virus mutant juste au moment où la pandémie a commencé. Image
Image
Read 60 tweets
De teleconferentie van 1 februari 2020 over de origine van #SARSCoV2: de puzzelstukjes vallen bijna allemaal op zijn plaats.

Ik waarschuw maar vast: dit wordt waarschijnlijk de langste twitterdraad ooit die ik zal schrijven.

eu.usatoday.com/story/opinion/…
De conferentie waar @MarionKoopmans, Ron Fouchier en @c_drosten een beslissende rol speelden in het naar voren schuiven van de zoönosetheorie.

Met grote dank aan @JamesCTobias voor het losweken van de notulen van deze conferentie...
...en de erop volgende e-mails van de verschillende deelnemers.

Deze waren tot voorheen zwartgelakt en dus was onbekend wat de deelnemers aan argumenten voor en tegen de #zoönosetheorie en #lableaktheorie waren.

Dat weten we nu wel.
Read 111 tweets
#Shi & her 2022 #China Patent: BALB/c mouse adapted strain of #SARS related coronavirus WIV1-RsSHC014S and application thereof..
Inventors: Shi Zhengli, Lin Haofeng, Liu Meiqin, Jiang Rendi
Zhou Peng. #WIV1 #RsSHC014S Image
The invention relates to the technical field of coronavirus, in particular to a BALB/c mouse adapted strain of SARS-related coronavirus WIV1-RsSHC 014S. Image
The SARS-associated coronavirus #RsSHC014 can effectively replicate and propagate in a corresponding cell line and a transgenic mouse by using #hACE2 as a receptor, Image
Read 62 tweets
If you're wondering why so many Bat coronaviruses seem to emerge from one research group in China, this paper provides a clue.

pubmed.ncbi.nlm.nih.gov/26920708/

It involves our usual friends... and a "novel" bat coronavirus called 4991 Image
There is just a little itty bit of a problem.
In "discovering" Bat Cov-4991 they didn't actually discover a virus.
More likely, they made one. Image
You see, they published complete gene sequences of the "bat viruses" in their paper, including spike sequences.

But not 4991.
Oh no, they didn't happen to have the complete sequence of that one. Just the RdRp.

ncbi.nlm.nih.gov/nuccore/KU3432…

@Daoyu15 ImageImage
Read 9 tweets
52. Update - @quay_dr exposes more RaTG13 fraud

The seminal paper from WIV claiming SARS-CoV-2 probably originated in bats appears to contain a contrived specimen, incomplete & inaccurate genomic assembly, & the signature of lab-derived synthetic biology

zenodo.org/record/5778318…
54. New #RaTg13 paper from @stvevenemassey

Exclusive to #DRASTIC

SARS-CoV-2's closest relative, RaTG13 was generated from a bat transcriptome not a fecal swab

Implications for the origin of COVID-19

drasticresearch.org/2021/11/23/sar…
Read 5 tweets
1. New #RaTg13 paper from @stvevenemassey

Exclusive to #DRASTIC

SARS-CoV-2's closest relative, RaTG13 was generated from a bat transcriptome not a fecal swab

Implications for the origin of COVID-19

drasticresearch.org/2021/11/23/sar…
2. Three explanatory threads by @stevenemassey

Evidence for ‘live’ RaTG13 at WIV, Part 1



Evidence for ‘live’ RaTG13 at WIV, Part 2



Evidence for ‘live’ RaTG13 at WIV, Part 3

3. Abstract
Read 8 tweets
One last thread on the origins of #SARSCoV2 before the upcoming publication of the US intelligence report. First of all, there are two things that might be even more important than the origin itself: research ethics and scientific debate. 1/
Since the beginning, some scientists working in Chinese institutions haven't been fully transparent. The history of #RaTG13 and the issues with its sequencing data, the miners pneumonia, the missing database, the pangolin CoV data. Hiding info, playing with words. Bad science. 2/
The second thing that I didn't like was the suppression of an open dialogue between scientists, which lasted for several months. Now it's easier for scientists to freely talk about the different scenarios, but do you remember how difficult it was one year ago? 3/
Read 11 tweets
The study identifying #RaTG13 was partially supported by NIH grant:R01AI110964 (link.springer.com/article/10.100…). US taxpayers' money was used for collecting/studying coronavirus samples from Mojiang mineshaft! @congressdotgov has the right/obligation to investigate this! @USRightToKnow Image
Congress Letter to NIH on taxpayer-funded research at the Wuhan Institute of Virology. @R_H_Ebright @TheSeeker268 @Ayjchan @BillyBostickson @jbloom_lab @ydeigin @Rossana38510044 scribd.com/document/49570…
Read 39 tweets
Question for bioinformaticians! Believe it or not, the first genome of bat virus #RaTG13 had some nucleotides that were supported by zero reads: in other words, the genome didn't match the sequences that were used to assemble it. How is it possible? 1/
#originsofSARSCoV2
Assemblers are computer programs, they do not "invent" nucleotides. So, what happened here? My explanation was that this genome was assembled using other reads, maybe in addition to the ones that were uploaded. 2/
In that case, I expected the authors to upload the missing reads.. Instead they did the opposite: they updated the genome, replacing the nucleotides supported by zero reads with the correct ones. So, the question is: how the heck did they assemble the genome the first time? 3/
Read 5 tweets
Some "conspiracists" always get it right!

Conspiracist, January 2020: "#RaTG13 virus looks like BtCoV/4991 described in an old paper that you didn't cite!" 📄

Average scientist, January 2020: 😴

Shi Zhengli, July 2020: "Yes, it was the same virus."

#SARSCoV2 #SARS2origins

1/
Conspiracist, May 2020: "Look, these sequences in database are dated 2017/2018. So you didn't sequence RaTG13 this year, as you wrote in the paper!" 🔬

Average scientist, May 2020: 😴

Shi Zhengli, July 2020: "Yes, actually we got the sequence in 2018."

2/
Conspiracist, May 2020: "Have a look at these Chinese theses we found on the internet: RaTG13 was found in a #mineshaft were people died of #pneumonia!" 😱

Average scientist, May 2020: 😴

Shi Zhengli, June 2020: "Oh yes we found it there, but.. well, the cause was a fungus"

3/
Read 8 tweets
La virologa cinese #LiMengYan dice che #SARSCoV2 è stato creato in un #laboratorio di #Wuhan. Senza vedere le prove (che lei sostiene di avere), mi risulta difficile credere alla sua tesi. 1/
nypost.com/2020/09/11/chi…
La scienziata, infatti, sostiene che #SARS2 sia una #chimera dei virus dei #pipistrelli ZC45 e ZXC21.

Ora, è vero che a Wuhan facevano chimere a scopi di ricerca, ma questi due virus sono troppo diversi da SARS2 per essere i suoi antenati diretti. 2/

Il nuovo #coronavirus infatti ha una similarità dell'87% con questi due virus, troppo poco per essere il risultato di una loro ricombinazione (il virus più simile a SARS2 è #RaTG13). 3/
Read 6 tweets
RaTG13. A thread.
RaTG13, the Bat-Cov most similar to Sars-Cov-2, was conjured out of the hat in late January 2020. The sample was allegedly collected in 2013, though submitted to NCBI only on 27-Jan-2020 by ... Zheng-Li Shi. |1
#Coronavirus #covid19 #sarscov2 #CoronavirusFacts
On 03-Feb-2020 RaTG13 was presented in an Article published by Nature, received on 20-Jan-2020 , one week before RaTG13 was submitted to NCBI GenBank. |2
nature.com/articles/s4158…
I guess this is the reason why RaTG13 was invented. No one would have bought the story of a bat origin when the sequence identity is ~80%. |3
Read 13 tweets
"The live SARS-like coronavirus SL-CoV-WIV1 has been isolated for the first time from bat droppings; and such virus has been confirmed to invade the host cells through the ACE2 of human beings..." ImageImageImage
Bat Severe Acute Respiratory Syndrome-Like Coronavirus WIV1
Encodes an Extra Accessory Protein, ORFX, Involved in Modulation of the Host Immune Response. (2016) #ZhengLiShi #PeterDaszak #EcoHealth
Funding: #NIAID 110964 ImageImageImageImage
In this study, we have developed a fast and cost-effective method
for reverse genetics of coronaviruses by combining two approaches developed by others..As the genomes can be divided into multiple short fragments, mutations can be introduced into individual
fragments easily. ImageImageImageImage
Read 68 tweets

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