Discover and read the best of Twitter Threads about #SingleMolecule

Most recents (3)

#quantum is here! Why not collect #fluorescence #photon-by-photon in your laser-scanning #microscope? We developed the #BrightEyes_TTM, an #opensource data acquisition system able to do it!
nature.com/articles/s4146…
@IITalk @ERC_Research @RNA_iit @MSCActions #BrightEyes_ERC
(1/5) Image
Our time-tagging #DAQ module can collect single-photon signals from 25 independent channels and reference signals from 3 channels. We showed how this module combined with a #SPAD array allows us to perform #FLISM and #FLFS. (2/5)
#imagescanningmicroscopy #fluorescence #lifetime Image
We characterized the #BrightEyes_TTM. We can reach a single-shot precision of 30 ps, 4 ns dead-time, and an "infinite" range in all channels, with a total flux of up to 125 MHz. (3/5)
#TCSPC #antibunching #quantum #microscopy #FPGA
#coding #LIDAR Image
Read 5 tweets
🚨 Interested in single-molecule imaging approaches and/or pore forming proteins? Check out this awesome work from Conall, James and Till at UNSW. Buckle up... 🧵

#UNSW @SingMolSci @Mol_Machines #SingleMolecule #imaging @PratoPores @_DrCJM_ @m_dunstone

doi.org/10.1101/2021.1…
Hold up... if you haven't already done so - follow @Mol_Machines to keep up to date with their awesome single-molecule studies :)
In essence, the study uses #TIRF microscopy to extract #kinetic information, from movies like the one below, for the entire assembly pathway of the pore-forming protein, PFO. What might look like the night sky disappearing, is actually hundreds of independent pore-forming events.
Read 21 tweets
tinyurl.com/yw6sk5uz
1/9 Could graphene coverslips improve your single-molecule fluorescence and superresolution experiments? In our article in @AdvancedAms we combine #DNAorigami with #graphene to perform #singlemolecule #biophysics, #biosensing & #superresolution microscopy.
2/9 Graphene is almost transparent and quenches fluorescence in a range of ~40 nm. Fluorescence intensity or fluorescence lifetime of single molecules directly tell you the distance of a molecule to the surface. Differences in the z-direction as small as 2.9 nm are resolved. Image
3/9 Dynamics with graphene energy transfer (GET). We use GET to visualize motion on top of a graphene surface and determine the timescale of fluctuations via autocorrelation analysis. Image
Read 9 tweets

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