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On @biorxivpreprint we report a direct TR-FRET-based protein quantification strategy (<1 h from lysis➡️read) in unmodified cell lines. If you're into PROTACs, and you hate running Westerns, check it out! Mini-thread: (1/n)
biorxiv.org/content/10.110…
Two birds with one stone: using clever biochemistry principles and a single POI-targeting antibody, we can rapidly & quantitatively measure both endogenous target engagement and protein levels in whole cell extracts, here exemplified for BRD4 (2/n)
A conventional blood-sweat-and-tears Western blot takes the average scientist 2 days. With our strategy, we initially optimized the assay in a 24-well plate format. The readout takes <1 h. Comparing BRD4 levels measured by our platform to Western blot were nearly identical (3/n)
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