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“Cryo-ExM” or “how to see (almost) everything you ever dreamt of”
Want to know more ?!? Check our story out with @CentrioleLab @nikolai_klena in @naturemethods: nature.com/articles/s4159… which combines cryo-fixation/Freeze substitution with #expansion #microscopy (#UExM).
1/Cryo-fixation, as demonstrated in electron microscopy, outperformed chemical fixations on subcellular structure such as the endoplasmic reticulum
2/Cryo-fixation circumvent the artifact of chemical fixations such as loss of antigenicity as seen with the mitochondrial outer membrane marker TOMM20
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