Discover and read the best of Twitter Threads about #bios

Most recents (1)

#PlantScienceClassics #11: The GUS reporter system. In 1987 Richard Jefferson & colleagues from @michaelbevan565's lab published the first reporter system to monitor promoter activity in planta in the @embojournal: doi.org/10.1002/j.1460… Title page of the paper showing its title “GUS fusions: be
In the 1980s it was possible to express transgenes in cells of different organisms, but visualizing & assaying gene expression was still problematic. The lacZ β-galactosidase was a commonly used reporter, but compromised due to endogenous enzymes breaking down its substrates too.
So Jefferson developed the Escherichia coli uidA-encoded β-glucuronidase (GUS) reporter during his PhD in David Hirsh’s lab @CUBoulder. Adding a GUS-substrate to Caenorhabditis elegans carrying the GUS-reporter led to a colorful precipitate in all tissues with GUS expression. A wild type Caenorhabditis elegans worm and one carrying the
Read 13 tweets

Related hashtags

Did Thread Reader help you today?

Support us! We are indie developers!


This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3.00/month or $30.00/year) and get exclusive features!

Become Premium

Too expensive? Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal Become our Patreon

Thank you for your support!