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Over the moon to share @danzhanghappy et al’s #preprint “Opposite polarity programs regulate asymmetric subsidiary cell divisions in grasses” - we identified a novel, distal polarity domain formed by the POLAR homolog in #Brachypodium @biorxiv_plants biorxiv.org/content/10.110…🧵/1
We transcriptionally profiled the unique bdmute phenotype–viable but no stomatal subsidiary cells (SCs)–to identify genes associated with SC formation -> 4th most downregulated gene = BdPOLAR!! /2 
CRISPRed bdpolar mutants showed defective SC divisions, much like classical polarity mutants that affect subsidiary mother cell (SMC) polarity factors like PANGLOSS1 homologs. Indeed, also bdpan1 mutants show this defect and double (polar;pan1) has 80% (!) defective SCs /3
