Discover and read the best of Twitter Threads about #cancerGenomics

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Finally the second chapter of my #PhD #thesis is out 💪🥳
> 1 year after thesis submission!
Thanks so much 🙏 to my co-authors @CFlensburg @ianjmajewski and Terry Speed for their persistence and support!
(thread 👇) #RNAseq #CancerGenomics #mutations
bmcbioinformatics.biomedcentral.com/articles/10.11…
Our goal was to decide on the library size needed to sequence a cohort of ~90 in-house Leukaemia RNA-Seq samples to allow good sensitivity for both DE analyses and mutation calling.
We used publicly available deeply sequenced RNA-Seq samples (from Leucegene) from the same cancer type and called mutations using 6 methods (combinations of 3 popular callers and filtering methods).
We called mutations on the initial and randomly downsampled data.
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