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1/11: Interested in genetic screening in #organoids? Check out #CRISPRLICHT 💡, our method for loss-of-function screening in cerebral organoids 🧠 out now in @ScienceMagazine. By @christopher_esk and @DLindenhofer. Short thread.
science.sciencemag.org/content/early/…
2/11: Screening in organoids is difficult because just like animal tissue - but unlike 2D cells - cerebral organoid tissue grows unequally, masking potential loss of function effects.
3/11: To overcome this problem, we developed a #CRISPR screen coupled to massive parallel lineage tracing with exact cell counting using dual barcoding in human cerebral organoids.
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