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A thread on how we determined 12 sub-3 Å spike-Fab #cryoEM structures from a single microscopy session❗️ Using #EPUMultigrid, we performed high-throughput epitope mapping for a panel of neutralising antibodies targeting the #SARSCoV2 spike protein 🧵
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thermofisher.box.com/s/a8mws76g5ew8…
The inspiration for this project came from our previous observation that the 6P-stabilised #SARSCoV2 spike (@McLellan_Lab) could produce ~3 Å structures from less than 2 hours of data collection👇
Over the last two years, through trial and error and searching the literature, we perfected the recipe for reproducible spike-Fab complexes: 24 μM 6P spike + 150 μM Fab + 0.01% fluorinated octyl maltoside (added just before plunging). 🧑🍳
