Discover and read the best of Twitter Threads about #flowcytometry
Most recents (4)
For anyone doing #flowcytometry, would you like to have a new protocol that reduces your #antibody costs by ~10-fold and also gives you higher quality data, with better signal-to-noise ratio?
Yes? Then read work by @olivertburton and @CarlyEWhyte. 1/6
…rentprotocols.onlinelibrary.wiley.com/doi/epdf/10.10…
Yes? Then read work by @olivertburton and @CarlyEWhyte. 1/6
…rentprotocols.onlinelibrary.wiley.com/doi/epdf/10.10…
Bottom-line-up-front: stain overnight. Antibody staining is sensitive to both dilution and time, so a titration that gives crummy staining in 30' can actually give beautiful staining overnight. The best part is, that lower dilution gives less background, for better separation 2/6
You can see this quite clearly with even tricky staining, like #Foxp3 and #cytokines. The overnight stain gives a stronger positive signal without increasing the background at all. Plus your cells are ready in the morning, when the cytometer is free, rather than 8pm at night! 3/6
I told my family from age 7 that I wanted to be a zoologist.
I am so proud of myself for all I have achieved so far and am grateful for life's opportunities everyday.
Our STEM journeys are important and I am excited to share mine with you.
I am so proud of myself for all I have achieved so far and am grateful for life's opportunities everyday.
Our STEM journeys are important and I am excited to share mine with you.
I graduated in BSc (hons) Zoology from @AngliaRuskin in 2015.
I struggled, mostly with exams.
After three years, I convinced myself I would never be smart enough to pursue any post-graduate studies so I got a job at @sangerinstitute as an animal tech 🐭
I struggled, mostly with exams.
After three years, I convinced myself I would never be smart enough to pursue any post-graduate studies so I got a job at @sangerinstitute as an animal tech 🐭
I immediately fell back in love with science.
I realised that commitment and enthusiasm were just as valuable as being able to take an exam.
I learnt so much from so many and before I knew it, I was looking up papers in the evening just to better contribute to the conversations
I realised that commitment and enthusiasm were just as valuable as being able to take an exam.
I learnt so much from so many and before I knew it, I was looking up papers in the evening just to better contribute to the conversations
Basics of #flowcytometry tweetorial!☺️
As promised @DrGeeONE @DrPayelMondal @siti_richardson @nusrat_xahra @seyma_buyucek @Gagandeepk5MD @RubyMughal2 @anjuthevirgo @anueru432 @kis_lorand @AlanPath @Pathology36 😁
1/11
#FlowCytomtery #HemePath #Flow #PathTwitter
As promised @DrGeeONE @DrPayelMondal @siti_richardson @nusrat_xahra @seyma_buyucek @Gagandeepk5MD @RubyMughal2 @anjuthevirgo @anueru432 @kis_lorand @AlanPath @Pathology36 😁
1/11
#FlowCytomtery #HemePath #Flow #PathTwitter
What is a Flow Cytometry?
💧Flow= fluid
💧Cyto= cells
💧Metry =measurement
Flow cytometry is a process of performing measurements on cells (or particles) that are in liquid suspension-multiple characteristics gets measured simultaneously as cell passes through light beam 2/11
💧Flow= fluid
💧Cyto= cells
💧Metry =measurement
Flow cytometry is a process of performing measurements on cells (or particles) that are in liquid suspension-multiple characteristics gets measured simultaneously as cell passes through light beam 2/11
A Brief History of Flow Cytometry:
The first invention of a coulter counter was in 1949 by Wallace H. Coulter- method for detecting and counting particles suspended in a fluid medium
Later named the Coulter principle.
3/11
#beckmancoulter
beckman.com/about-us/compa…
The first invention of a coulter counter was in 1949 by Wallace H. Coulter- method for detecting and counting particles suspended in a fluid medium
Later named the Coulter principle.
3/11
#beckmancoulter
beckman.com/about-us/compa…
I am really thrilled to release #AutoSpill onto @biorxivpreprint. It is a novel method for applying compensation to #flowcytometry data, which reduces the error by ~100,000-fold. It is thanks to AutoSpill that we can push machines to their max colours
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
#AutoSpill is a beautiful example of what #maths can add to #immunology, led by the talented Dr Carlos Roca.
It has really open up my eyes to the potential of #computationalbiology, and is the reason why we have a new #datascience position available:
babrahaminstitute.livevacancies.co.uk/#/job/details/…
It has really open up my eyes to the potential of #computationalbiology, and is the reason why we have a new #datascience position available:
babrahaminstitute.livevacancies.co.uk/#/job/details/…
So how does #AutoSpill work? If you just want to compensate your data, simply upload your single colour controls to autospill.vib.be and then copy the spillover matrix to your #flowcytometry program of choice
@CarlyEWhyte can walk you through the whole process in <2'
@CarlyEWhyte can walk you through the whole process in <2'
