Discover and read the best of Twitter Threads about #gastruloids
Most recents (5)
We are extremely happy to present our lab’s first #preprint! Together with @teamstembryo we identify #hypoxia as a major microenvironmental factor that governs ES cell lineage commitment.
biorxiv.org/content/10.110…
Short summary 👇🏼👇🏼 (1/11)
biorxiv.org/content/10.110…
Short summary 👇🏼👇🏼 (1/11)
Have you ever wondered how environmental factors such as #hypoxia together with intrinsic cellular regulators shape stem cell identity and differentiation capacity? We have (a lot!). But wait a minute you say, isn’t oxygen essential for mammalian life? (2/11)
Of course it is! But… before placentation, the embryo develops in an increasingly hypoxic environment where oxygen tension is as low as 1.5%. But what’s the physiological relevance? @odedrechavi
(3/11)
(3/11)
Our study “An in vitro model for anteroposterior organisation during human development” is out! nature.com/articles/s4158… @nature @Cambridge_Uni @_Hubrecht A summary: (1/10)
In this study, we used human Embryonic Stem Cells that we disassociate and resuspend together into small, spherical 3D aggregates. Remarkably, these are able to spontaneously break symmetry, and, over 3 or 4 days, they elongate along a single anterior-posterior axis (2/10) 
Using a triple-coloured reporter of the 3 germ layers, we saw that these aggregates had polarised expression of Brachyury at the most distal end, and they express all three fluorescent reporters in an organised manner (3/10)
#BSDBGenSoc2020 final part
The results show that gastruloids are organized and that this organization corresponds to that of the embryo; see go.nature.com/3d0lB6b for details.
One surprising observation here is the differentiation trajectories of NMPs and, in particular the gene expression sequence of somitogenesis: Bra ->Tbx6 -> Uncx4.1.
#BSDBGenSoc2020 These days we all have other things in our minds, it is difficult to concentrate and many of my thoughts R w/ family anf friends in #Spain) but discussing our work, is an important part of our being and it is going to be important in the weeks and months to come.
While many, all, of us rather do al this in person, we are going to have to adjust in the weeks to come. #BSDBGenSoc2020 is a good way to explore how we are going to do it so, THANKS @_BSDB_ and @the_Node for organizing and running this.
My Tweetorial follows the phenomenal one from @TimFulton_1 @SteventonLab (with whom @AMA_Lab work closely) this morning and is very related.
And now onto the tweetorial in the form of a loooong thread.
And now onto the tweetorial in the form of a loooong thread.
We’re proud to present our #preprint, where we induce embryo-like morphogenesis in #gastruloids, resulting in the generation of trunk-like-structures (#TLS) with #somites flanking the #neuraltube @MPI_MolGen w/ @adrianobolondi . A short summary (1/12) biorxiv.org/content/10.110…
#Gastruloids are a fantastic culture system, but lack proper morphogenesis, e.g. neural cells do not form a neural tube and presomitic mesoderm does not condense into somites. Can we induce proper embryo-like morphology by mechano-chemical manipulation? Yes, we can! (2/12)
Embedding 96h #gastruloids in 5% #matrigel resulted in trunk-like-structures, with somites flanking the neural tube. Activation of WNT signaling, either alone or in combination with BMP inhibition lead to hyper-epithelialized somites arranged as a bunch of grapes (3/12)
