Discover and read the best of Twitter Threads about #invivo

Most recents (5)

🧵When resources are limited, the question of what knowledge is needed and when — the ‘decision gate’ analogy — becomes the principal strategic consideration in determining the priority of activities during early drug development
@NatRevDrugDisc #biotech…
Evidence showing ligands & receptors to be variable and discerning instigators of signals, and translators of information, respectively, have illustrated the fallacy of assuming that the surrogate host cell is an inert vessel-Terry Kenakin @NatRevDrugDisc…
Stepping back from the rather safe HTS paradigm to discovery in virtual space which is still not yet fully developed, certainly needs courageous management decisions not only in terms of financial investments, but also in organizational evolution @Aalanine…
Read 32 tweets
Multiplication in a single neuron. In a new @Nature article, @MalisJonatan, @BirteZuidinga, Axel Borst, and I describe the arithmetic of #Drosophila motion vision in biophysical terms. #Neuroscience @MPIforBI @MSCActions @EMBO (1/4)…
Using #PatchClamp #Ephys #InVivo, we recorded the membrane potentials of ON motion-sensing T4 neurons and their presynaptic partners. One type of input neuron (blue) provides continuous shunting inhibition. (2/4)
Only during visual motion in a T4 neuron’s preferred direction, well-timed release from inhibition amplifies coincident excitation in a multiplication-like fashion. Such ‘multiplicative disinhibition’ can approximate a logic AND gate. (#FlyPushing by @BirteZuidinga; 3/4)
Read 5 tweets
#SciTwitter After a lot of research and asking around, I'm making the lab equipment recommendations 🧵 I wish I'd had 2 months ago. RT/share with a #newPI or startup 🔬⚗️🛒
Note, much of the equipment hasn't arrived yet, will add comments after actual use.
-80 #freezer
Two clear winners: PHC (@panasonic) and Stirling Ultracold. Both low energy, quiet, reliable. We went with PHC because I know those to last many years, and slightly cheaper.
Thanks @MarcoJost_ @letUbeU @aryelipman #MBCBiolabs
-20 #freezer
Less clear, many viable options. We ended up getting a split of PHC MDF -30 (recommended as quieter) and much cheaper Corepoint Scientific/@VWR, will see which we prefer. Thermo hasn't failed #MBCbiolabs, but $$$ and several people said poor customer support.
Read 14 tweets
"The live SARS-like coronavirus SL-CoV-WIV1 has been isolated for the first time from bat droppings; and such virus has been confirmed to invade the host cells through the ACE2 of human beings..." ImageImageImage
Bat Severe Acute Respiratory Syndrome-Like Coronavirus WIV1
Encodes an Extra Accessory Protein, ORFX, Involved in Modulation of the Host Immune Response. (2016) #ZhengLiShi #PeterDaszak #EcoHealth
Funding: #NIAID 110964 ImageImageImageImage
In this study, we have developed a fast and cost-effective method
for reverse genetics of coronaviruses by combining two approaches developed by others..As the genomes can be divided into multiple short fragments, mutations can be introduced into individual
fragments easily. ImageImageImageImage
Read 68 tweets
BEFORE READING THIS THREAD, do you think this statement is TRUE or FALSE?

Despite being a documented #epidemic for decades, @HHSGov's #CenterForDiseaseControl(@CDCgov) intentionally obstructs its collecting #statistics on what causes at least 10% of deaths NEEDLESSLY every year.

HINT: You already know of these systemic #DataScams due to #PublicCorruption & #RegulatoryCapture:

🤥#UScensus is maliciously biased

Thread coming soon about #CauseOfDeath #DataFraud

#AmericanSwamp #MedicalControlFraud

ANOTHER HINT: My inability to escape intrahospital, interhospital & health/disability insurers’ #ConspiracyAgainstRights using #MedicalControlFrauds exposes motive of the right answer.

Watch this video later to understand #PublicCorruption by example📽️
Read 43 tweets

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