Discover and read the best of Twitter Threads about #koralovlab

Most recents (1)

Running (EC)CITE-seq? #scRNAseq

Can I use FACS staining protocol? Should #CITEseq Abs be used at saturating conc? Can I reduce staining volume? Does cell# at staining matter? How can I minimize Background?

Tweetorial of biorxiv.org/content/10.110…
@SBKoralov @skinUCPH @NYGCtech
When setting up #ECCITEseq we encountered many questions that we could not find answers to. Figuring we were not alone, we tested several conditions (tissue/conc./volume/cell #) and compiled the results into a manuscript that we hope will assist others!

biorxiv.org/content/10.110…
We find that staining with high antibody conc. can cause unnecessarily high background signal and that concentrations of most antibodies can be reduced beyond general recommendations. Titration is best, but 0.6-1 µg/mL range appears to be a good starting point for most Abs tested
Read 9 tweets

Related hashtags

Did Thread Reader help you today?

Support us! We are indie developers!


This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3.00/month or $30.00/year) and get exclusive features!

Become Premium

Too expensive? Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal Become our Patreon

Thank you for your support!