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Running (EC)CITE-seq? #scRNAseq
Can I use FACS staining protocol? Should #CITEseq Abs be used at saturating conc? Can I reduce staining volume? Does cell# at staining matter? How can I minimize Background?
Tweetorial of biorxiv.org/content/10.110…
@SBKoralov @skinUCPH @NYGCtech
Can I use FACS staining protocol? Should #CITEseq Abs be used at saturating conc? Can I reduce staining volume? Does cell# at staining matter? How can I minimize Background?
Tweetorial of biorxiv.org/content/10.110…
@SBKoralov @skinUCPH @NYGCtech
When setting up #ECCITEseq we encountered many questions that we could not find answers to. Figuring we were not alone, we tested several conditions (tissue/conc./volume/cell #) and compiled the results into a manuscript that we hope will assist others!
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
We find that staining with high antibody conc. can cause unnecessarily high background signal and that concentrations of most antibodies can be reduced beyond general recommendations. Titration is best, but 0.6-1 µg/mL range appears to be a good starting point for most Abs tested
