Discover and read the best of Twitter Threads about #lightsheet

Most recents (4)

How do you image the large and the small at the same time? We developed new 🔬 technology to image centimeter-scale specimens - including whole mouse brains 🧠 - with diffraction-limited resolution and without sectioning. #mesoscale #imaging

biorxiv.org/content/10.110…

🧵 (1/n)
ExA-SPIM is a technology based on innovations in (1) tissue processing and (2) large-scale microscopy 🔬. Our team developed new methods for expansion for microscopy (ExM) of large tissue volumes. #teamscience #expansionmicroscopy

protocols.io/view/whole-mou…

🧵 (2/n)
By combining ExM with a new SPIM system based on technologies from electronics metrology, ExA-SPIM pushes past the volumetric imaging barrier 🚧 that constrains previous imaging approaches. (inspired by recent plot from @Daetwyler_St, @RetoPaul) #lightsheet #microscopy

🧵 (3/n)
Read 12 tweets
The CompassLSM: an #opensource #mesoSPIM-inspired #lightsheet #Microscope excitation path that allows 3.5 um axial resolution over a 10 mm FOV using axially scanned lightsheet microscopy osapublishing.org/boe/fulltext.c…
My main comment about this paper is about publication strategy and not science: I probably should not have called it #opensource: "CAD file available upon request" - it should be easier than that! Don't make it so hard for biologists to copy your work!
If you want your technology adopted, make adoption really, really easy - find a vendor for parts kits, provide the complete parts list, provide software, provide documentation - don't let users do a lot of the legwork. Lowering the barrier to entry is key.
Read 8 tweets
We just updated our preprint, reporting a novel single-objective #lightsheet design that is capable of imaging large samples, with a large imaging volume, uncompromized image resolution and speed, and multi-view imaging. (1/9)

doi.org/10.1101/2020.0…
Thanks to @DrLachie, now we have a visual abstract which explains how the microscope works. First half of the movie below. (2/9)
Second half of the visual abstract by @DrLachie (3/9)
Read 9 tweets
We are excited to share the mesoSPIM, a versatile open-source light-sheet microscope for imaging in cleared tissue. #mesoSPIM @openSPIM #lightsheet
Check out our webpage: mesospim.org
The mesoSPIM is compatible with all clearing techniques and was tested with
active and passive CLARITY, CUBIC, BABB, iDISCO and MASH.
The key mesoSPIM feature is axially scanned light-sheet microscopy (ASLM) introduced by @RetoPaul and @kD3AN which allows uniform axial resolution across large FOVs. To translate the light-sheet, we are using tunable lenses from
@optotunecompany
Read 14 tweets

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