Discover and read the best of Twitter Threads about #microseq2021
Most recents (1)
I've just released (during #MicroSeq2021) a new short-read polishing tool for fixing errors in long-read bacterial genome assemblies: Polypolish!
github.com/rrwick/Polypol…
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github.com/rrwick/Polypol…
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There are many other short-read polishing tools, including HyPo, NextPolish, ntEdit, Pilon and POLCA. So what does Polypolish do differently to warrant another?
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Most other polishers use 'normal' short-read alignments, where each read is aligned to one best location (randomly chosen in a tie). This works fine in non-repeat sequences, but errors in repeats often lead to a lack of alignments and therefore can't be fixed.
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