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Excited to share our new preprint on "Assessment of 3D MINFLUX data for quantitative structural biology in cells"
with @AlistairCurd
biorxiv.org/content/10.110…
We thank @LSchermelleh @HohlbeinLab @michellepeckham for the feedback and looking forwards to yours!
#MINFLUX
with @AlistairCurd
biorxiv.org/content/10.110…
We thank @LSchermelleh @HohlbeinLab @michellepeckham for the feedback and looking forwards to yours!
#MINFLUX
Thanks, @Stefan_W_Hell for sharing the data which helped to revisit the claims made in the following article "MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells" @naturemethods
nature.com/articles/s4159…
Hope our findings are useful for MINFLUX research!
nature.com/articles/s4159…
Hope our findings are useful for MINFLUX research!
First, using PERPL, our localization precision estimates of 0.98±0.02 nm, 3.20±0.05 nm and 3.31±0.08 nm broadly agreed with the published analysis but implying FWHM of 2.4-7.1 nm.
So, what should be the best theoretical resolution? 1-3 nm as claimed?
So, what should be the best theoretical resolution? 1-3 nm as claimed?
Hey #STED, #MINFLUX people,
To continue the public discussion, I list here some of the points I made during my talk.
Summary: MINFLUX is a promising technology but at an early stage of development esp for biological research.
Thread👇
cc @Stefan_W_Hell @BalzarottiFran
To continue the public discussion, I list here some of the points I made during my talk.
Summary: MINFLUX is a promising technology but at an early stage of development esp for biological research.
Thread👇
cc @Stefan_W_Hell @BalzarottiFran
Disclaimer 1: I haven't seen or worked with #MINFLUX setup, my arguments mainly on application-side
Disclaimer 2: I asked these questions via email (Sept 2020) and requested raw data and codes but haven't received a reply yet
I hope @rita_strack @naturemethods can help!
Disclaimer 2: I asked these questions via email (Sept 2020) and requested raw data and codes but haven't received a reply yet
I hope @rita_strack @naturemethods can help!
You can learn more about #MINFLUX from this excellent talk by @Stefan_W_Hell
Also, some papers making an effort in this direction
Also, some papers making an effort in this direction
